抗体功能化红色荧光纳米金刚石，100 nm Fluorescent Nanodiamond Goat AntiRabbit；~3 ppm NV; delivered in PBS with 0.1% BSA as a stabilizer at 1 mg/mL

Red Fluorescent Nanodiamond Functionalized with Antibodies

Biofunctionalized fluorescent nanodiamonds are infinitely photostable labels for fluorescent and multiphoton microscopy applicable to cellular tracking and long-term observation of biological processes in the same fields of view in live cell-imaging and fixed cells. Protein, antibody, biologically active organic molecules, and custom functionalizations are possible. We provide nanodiamonds with nitrogen-vacancy (NV) centers with red-to-near-infrared fluorescence, NVN centers with green fluorescence, and novel particles available only at Adamas with a combination of NV and NVN centers within a single particle with visual yellow fluorescence. Fluorescence from NV centers can be magnetically modulated providing a means to eliminate the fluorescent background by image subtraction and to significantly enhance the signal-to-noise ratio. This property can be very useful in future biosensing applications, for example in clinical diagnostics such as lateral flow assays (LFA). Relevant applications also include super-resolution microscopy, multiphoton microscopy, time-gated microscopy and correlative microscopy.

生物功能化荧光纳米金刚石是用于荧光和多光子显微镜的无限光稳定性标签，适用于活细胞成像和固定细胞中相同视野内的细胞跟踪和生物过程的长期观察。蛋白质、抗体、生物活性有机分子和定制功能化是可能的。我们提供具有红色至近红外荧光的氮空位 (NV) 中心的纳米金刚石、具有绿色荧光的NVN中心以及仅在Adamas [北实纵横科学公司] 提供的新型颗粒，其在单个颗粒中结合了NV和NVN中心，具有可见的黄色荧光。来自NV中心的荧光可以进行磁调制，从而提供一种通过图像减法消除荧光背景并显著提高信噪比的方法。该特性在未来的生物传感应用中非常有用，例如在临床诊断中，例如侧向流动测定 (LFA)。相关应用还包括超分辨率显微镜、多光子显微镜、时间门控显微镜和其他相关显微镜等。