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高分辨率尺寸排阻色谱树脂,Sephacryl S-200 HR

Sephacryl 高分辨率尺寸排阻色谱树脂允许在实验室和工业规模上通过尺寸排阻色谱快速且可重现地纯化蛋白质、多糖和其他大分子。17058410;17058401;17058405
货号(SKU): 17058401
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 26,004.06

Sephacryl High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.

经济高效的凝胶过滤树脂可提供高分辨率和快速简便的操作。
Sephacryl HR 系列提供了五种不同的分级范围,可在较宽的分子量范围内实现可重现的分离。
Sephacryl S-200 高分辨率树脂设计用于纯化抗体、血清蛋白和中等大小的蛋白质(分级范围为 5 × 10³ – 2.5 × 10⁵)。
BioProcess 树脂支持工业应用,并在批准的工艺中得到很好的证实。
Sephacryl 高分辨率色谱树脂是一种高度通用的凝胶过滤树脂,可提供广泛的分馏能力。 Sephacryl 高分辨率树脂的基质是烯丙基葡聚糖和 N,N'-亚甲基双丙烯酰胺的交联共聚物。这种交联提供了良好的刚性和化学稳定性。窄的粒径分布以及陡峭的选择性曲线导致了良好的制备特性并保持了高分辨率。树脂的亲水性最大限度地减少了非特异性吸附并最大限度地提高了回收率。出色的分辨率和流动特性、长期的物理和化学稳定性以及易于处理的特性使 Sephacryl HR 成为常规纯化的首选树脂。

Sephacryl High Resolution 提供一系列不同的散装尺寸和方便的预包装形式,便于放大和工艺开发。作为一种生物工艺树脂,Sephacryl High Resolution 通过供应安全和全面的技术和法规支持满足工业需求

Sephacryl S-100 High Resolution 设计用于分离肽段和小蛋白质(球状蛋白质的分级范围 1 × 10³–1 × 10⁵)。 Sephacryl S-200 High Resolution 用于纯化抗体、血清蛋白和中等大小的蛋白质(分级范围为 5 × 10³–2.5 × 10⁵)。 Sephacryl S-300 High Resolution 用于纯化抗体、血清蛋白和中等大小的蛋白质(分级范围为 1 × 10⁴–1.5 × 10⁶)。 Sephacryl S-400 高分辨率,用于分离多糖、具有扩展结构的大分子,甚至是质粒等小颗粒。 (分级范围球状蛋白质 2 × 10⁴–8 × 10⁶)。 Sephacryl S-500 高分辨率,可分离多糖、具有扩展结构的大分子,甚至是质粒等小颗粒(分离范围 Dextrans 4 × 10⁴–2 × 10⁷)

  • Cost-efficient gel filtration resins providing high resolution with fast and easy operation.
  • Five different fractionation ranges available in Sephacryl HR family allows for reproducible separations over a wide molecular weight range.
  • Sephacryl S-200 High Resolution resin is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³ – 2.5 × 10⁵).
  • BioProcess resin supported for industrial applications and well-established in approved processes.

Sephacryl High Resolution chromatography resins is a highly versatile gel filtration resins that offers a wide range of fractionation capabilities. The matrix of Sephacryl High Resolution resins is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the resins minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl HR the resin of choice for routine purification.

Sephacryl High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As a BioProcess resin Sephacryl High Resolution meets industrial demands with security of supply and comprehensive technical and regulatory support

Sephacryl S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 10³–1 × 10⁵). Sephacryl S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³–2.5 × 10⁵). Sephacryl S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 10⁴–1.5 × 10⁶). Sephacryl S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 10⁴–8 × 10⁶). Sephacryl S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 10⁴–2 × 10⁷)

 

ParameterSephacryl S-200 HR, 750 mL
BioProcess resinYes 
Particle size, d50V1~50 µm 
MatrixCross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide. 
Particle Size25 µm-75 µm 
pH stability, operational23–11 
pH stability, CIP32–13 
Pressure/flow characteristics4Pressure/flow characteristics: ≥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20°C using buffers with a viscosity similar to water).  
Fractionation range (Mr) (globular proteins)5 × 10³–2.5 × 10⁵ 
Fractionation (Mp) Dextrans1 x 10³-8 x 10⁴ 
Fractionation Range (Mr) DNA Exclusion Limit30 bp 
Exclusion limit (Mr) (globular proteins)~4 × 10⁵ (400 kDa) 
Storage4 to 30°C, 20% Ethanol 
Chemical stabilityCommonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl 
Certificate of AnalysisYes 
Pack size750 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 4The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16