MacroCap SP is a cation exchanger designed for the purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (i.e., PEGylated proteins) that are intended for use as biopharmaceuticals.
MacroCap SP 是一种阳离子交换剂，旨在分离聚乙二醇化蛋白质和其他大生物分子。它允许在高负载条件下以高选择性分离单、寡聚和非聚乙二醇化蛋白质。 MacroCap SP 基于丙烯酰胺-葡聚糖共聚物树脂，具有适用于大生物分子的传质特性。基础基质是高度多孔的，这为吸附聚乙二醇化蛋白质提供了高可用表面积。大孔径也使 MacroCap SP 适用于结合其他大蛋白质，例如。免疫球蛋白M。
MacroCap SP 有多种不同的散装尺寸和方便的预包装形式，便于放大和工艺开发。作为 BioProcess 树脂系列的成员，MacroCap SP 通过供应安全性以及全面的技术和法规支持满足工业需求。
- Strong cation exchanger for PEGylated proteins and other large biomolecules.
- High purity and yield of PEGylated proteins at high sample loads.
- Highly porous base matrix makes extensive surface area available for adsorption of large molecules.
MacroCap SP is a cation exchanger designed to separate PEGylated proteins and other large biomolecules. It allows separation of mono- from oligo- and non-PEGylated proteins with high selectivity under high load conditions. MacroCap SP is based on acrylamide-dextran copolymer resins with mass transfer properties suitable for large biomolecules. The base matrix is highly porous, which gives high available surface area for adsorption of PEGylated proteins. The large pore size also makes MacroCap SP suitable for binding other large proteins, e.g. IgM.
MacroCap SP is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, MacroCap SP meets industrial demands with security of supply and comprehensive technical and regulatory support.
|Parameter||MacroCap SP, 1 L|
|Chromatography technique||Ion Exchange Chromatography |
|BioProcess resin||Yes |
|Particle size, d50V1||~50 µm |
|Ion Exchanger Type||Strong cation exchanger |
|Matrix||Acrylamide-dextran copolymer |
|Ionic Capacity||0.10-0.13 mmol H+/ml medium |
|pH stability, operational2||4–11 |
|pH stability, CIP3||2–13 |
|Flow rate||< 120 cm/h in BPG 300, 20-cm bed height or 70 cm/h in BPG 300, 30-cm bed height using process buffers with the same viscosity as water at v 3 bar (0.3 MPa) |
|Storage||4 to 30°C 0.2 M Sodium Acetate in 20% Ethanol |
|Chemical stability||Commonly used aqueous buffers, 0.5 M NaOH, 0.1 M citric acid, 25% ethanol, 30% propanol, 30% methanol, 50% ethylene glycol, 1% Tween-20, and 1% SDS. |
|Certificate of Analysis||Yes |
|Pack size||1 L |