链霉亲和素-琼脂糖来源于阿维丁链霉菌，Streptavidin−Agarose from Streptomyces avidinii；buffered aqueous suspension

链亲和素琼脂糖用于蛋白质层析，亲和层析和重组蛋白表达和分析。链亲和素琼脂糖可以被用来研究烟草蚀纹病毒蛋白酶切割融合蛋白的定向固定。链亲和素琼脂糖被用来研发从天然植物提取物中筛选三重DNA结合物。

Streptavidin−agarose from Streptomyces avidinii has been used:

• to pull down biotinylated cell surface proteins during the quantification of plasma membrane transforming growth factor β (TGFβ) receptor II (TβRII) and Tβ
• RII internalization
• in biotinylated miRNA pull-down assay; as secondary antibodies in immunoprecipitation

用于纯化含有蛋白质或DNA结合蛋白质的生物素

 

Streptavidin is a homotetrameric protein, isolated from Streptomyces avidinii, which, like avidin, has a high affinity for biotin. Streptavidin is slightly anionic (pI ~ 5-6) and non-glycosylated. These properties contribute to its relatively low non-specific binding compared to egg white avidin. Streptavidin is also more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin-agarose can be used to immobilize or isolate various biotinylated macromolecules and complexes (proteins, antibodies, lectins, nucleic acids, receptors, and ligands). The inherent high-affinity streptavidin-biotin interaction requires harsh conditions to release biotinylated macromolecules. This feature makes streptavidin-agarose useful in a variety of affinity purification applications.

 

在pH7.2、含有0.05M NACl和0.02%叠氮化钠的0.01 M磷酸钠缓冲液中复溶