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链霉亲和素琼脂糖凝胶亲和树脂,Streptavidin Sepharose High Performance affinity resin

Streptavidin Sepharose HP是一种亲和树脂,用于生物素化蛋白质和其他生物分子的可靠和高分辨率纯化。
货号(SKU): 17511301
增值税发票 √ 免费送货 √ 订货电话:010-68689484
€704.49

Streptavidin Sepharose High Performance is an affinity resin for reliable and high-resolution purification of biotinylated proteins and other biomolecules.

高分辨率——由于珠尺寸为 34 µm。
多功能——任何生物素化配体均可用作亲和纯化的配体。
可用于利用与链霉亲和素的相互作用——生物素-链霉亲和素的强相互作用或 2-亚氨基生物素和链霉亲和素的弱相互作用。
生物素化蛋白质和其他生物分子纯化
链霉亲和素对生物素具有极高的亲和力和特异性。 Streptavidin 从 Streptomyces avidinii 中分离出来并固定在 Sepharose High Performance 上。 该树脂具有刚性、高度交联的珠状琼脂糖基质,具有高化学稳定性。

链霉亲和素与生物素之间的强相互作用可用于纯化抗原。 生物素化的抗体-抗原复合物与 Streptavidin Sepharose High Performance 结合,可以从中洗脱抗原。 另一种选择是利用 2-亚氨基生物素和链霉亲和素之间的相互作用,这是较弱的。

  • High resolution – due to 34 µm bead size.
  • Versatile – any biotinylated ligand can be used as a ligand for affinity purification.
  • Useful for exploiting interaction with streptavidin – the strong interactions of biotin-streptavidin or the weaker interactions of 2-iminobiotin and streptavidin.

Biotinylated protein and other biomolecule purification

Streptavidin has an extremely high affinity and specificity for biotin. Streptavidin is isolated from Streptomyces avidinii and immobilized on Sepharose High Performance. The resin possesses a rigid, highly cross-linked beaded agarose base matrix for high chemical stability.

The strong interaction between streptavidin and biotin can be used to purify antigens. Biotinylated antibody-antigen complexes bind to Streptavidin Sepharose High Performance, from which the antigen can be eluted. Another option is to exploit the interaction between 2-iminobiotin and streptavidin, which is weaker.

Compatible empty columns

Cytiva offers a variety of Life Sciences solutions for chromatography, including empty columns for packing your own columns. Streptavidin Sepharose High Performance is compatible for use with HiScale, Tricorn and XK columns.

Convenient prepacked columns

Streptavidin Sepharose High Performance is available in the following prepacked formats:

  • HiTrap Streptavidin HP columns: easy for use with a syringe, pump, or chromatography system.
  • Streptavidin HP SpinTrap: spin columns for use with a benchtop centrifuge
  • Streptavidin HP MultiTrap: 96-well plates for high throughput screening.
  • Streptavidin Mag Sepharose magnetic beads

Successful use in published research

Streptavidin Sepharose High Performance has been used in a variety of research applications, including:

 

ParameterStreptavidin Sepharose High Performance, 5 mL
Chromatography techniqueGroup specific affinity 
Particle size, d50V1~34 µm 
MatrixHighly cross-linked agarose, 6% 
Binding Capacity/ml Chromatography Medium>300 nmol biotin/mL resin 6 mg biotinylated BSA/mL resin 
pH stability, operational24–9 
pH stability, CIP32–10.5 
Flow velocity< 150 cm/h 
Storage4 to 8°C, 20% Ethanol 
Pack size5 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32