重组蛋白A琼脂糖凝胶FF抗体纯化树脂,rProtein A Sepharose Fast Flow antibody purification resin

rProtein A Sepharose 4 Fast Flow是一种亲和树脂,用于实验室和工艺规模的抗体纯化。17127903;17127904;17127906;17127902;17127905;17127907;17127901
货号(SKU): 17127904
增值税发票 √ 免费送货 √ 订货电话:010-68689484

rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.

在蛋白质 A 树脂中,它非常适合单克隆抗体 (mAb) 工艺。
rProtein A Sepharose 4 Fast Flow 建立在完善的 Sepharose Fast Flow 平台之上,并用于许多经批准的单克隆抗体工艺。它由与交联的 4% 琼脂糖基质偶联的重组蛋白 A 组成。高度交联的琼脂糖树脂可实现高流速和高生产率。

Protein A 树脂特性
重组蛋白在大肠杆菌中产生,并通过定向偶联进行工程化以增强结合能力。环氧基偶联确保低配体泄漏。重组蛋白对 IgG Fc 区的特异性与天然蛋白相似,具有高度的选择性和产量。


  • For monoclonal and polyclonal antibody purification at both laboratory and process scale.
  • Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
  • Free from animal-derived components.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Monoclonal and polyclonal antibody purification

rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.

Protein A resin characteristics

The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.


ParameterrProtein A Sepharose Fast Flow
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandRecombinant protein A (E.coli) 
Particle size, d50V1~90 µm 
MatrixCross-linked agarose, 4%, spherical 
Particle Size60 µm-165 µm 
Ligand Coupling MethodEpoxy activation 
Dynamic binding capacity, QB102~35 mg hIgG/mL resin 
Ligand density6 mg rProtein A/ml medium 
Total binding capacity3~50 mg hIgG/mL resin 
pH stability, operational43–10 
pH stability, CIP53–12 
Pressure/flow characteristics6150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water) 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol 
Certificate of AnalysisYes 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a 7.5/50 PEEK-column at 5 cm bed height (3 min residence time) for human IgG in 0.020 M NaH₂PO₄, pH 7.0
  • 3Human IgG in excess is loaded in 0.020 M NaH2PO4 at pH 7.0 on a 7.5/50 PEEK-column. The binding capacity is obtained by measuring the amount of eluted human IgG in 0.1 M glycine at pH 3.0.
  • 4pH range where resin can be operated without significant change in function
  • 5pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2. pH range where resin can be subjected to cleaning-in-place without significant change in function Reducing agent e.g. 100 mM 1-Thioglycerol followed by 15 mM NaOH is among the most efficient CIP for rProtein A sepharose Fast Flow”
  • 6The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16