重组人Raf-1蛋白激酶，Raf-1 Protein Kinase human；recombinant, expressed in E. coli, ~20 units/mg protein

Raf-1 is a serine/threonine protein kinase which is involved in the MAPK signal transduction pathway, a pathway that is involved in the development and progression of cancer. Raf-1 kinases are used to study potential cancer treatments as well as the responses to cancer treatments [1].

 

Raf-1 activates the protein kinases MEK1 and MEK2. MEK1 and MEK2 are phosphorylated to activate protein kinases ERK1 and ERK2. Activated ERKs play an important role in cell division, apoptosis, cell differentiation, and cell migration [2].

 

One unit will activate MEK B substrate by 10% per min at pH 7.0 at 30 °C.

 

Solution in 50 mM Tris-HCl, pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 10% glycerol.