邻苯二胺二盐酸盐，o-Phenylenediamine dihydrochloride [OPD]；peroxidase substrate

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

 

邻苯二胺是适用于 ELISA 法的过氧化物酶底物。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在 450nm 下通过分光光度计读取。可以用 3N HCl 或 3M H₂SO₄ 终止 OPD 反应，并在 492nm 处读取。

 

储存时颜色可能变深。