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软骨素酶AC来源于肝素黄杆菌,Chondroitinase AC from Flavobacterium heparinum;lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

别名:软骨素AC裂解酶;Chondroitin AC lyase; CAS号: 9047-57-8 EC 号: 4.2.2.5 (BRENDA, IUBMB) MDL编号: MFCD00130796 NACRES: NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): C2780
CAS号: 9047-57-8
MDL号: MFCD00130796
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说明

来自Sigma的软骨素酶AC已用于大规模制备糖胺聚糖(GAG)级分,用于研究硫酸真皮中的结构和序列基序。
软骨素酶AC已应用于商业样品(如膳食补充剂)中硫酸软骨素的分析。
来自肝素黄杆菌的软骨素酶AC是一种酶,通过消除机制裂解硫酸化和非硫酸化多糖链,其中己糖胺和葡萄糖醛酸残基之间具有(1-4)键。所得低聚糖产物主要是具有不饱和糖醛酸的二糖。软骨素酶AC专门降解硫酸软骨素A和C,但不降解硫酸软骨素B(硫酸真皮)。

应用

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
 

生化/生理作用

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
 

其他说明

Contains potassium phosphate buffer salts and BSA as stabilizer.
 

适用性

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
 

单位定义

One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
 

制备说明

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
 
 

属性

偶联物

(Glucosaminoglycan)

质量水平

200

形式

lyophilized powder

specific activity

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

组成

Protein, ~15% Lowry

溶解性

0.02 M phosphate buffer: soluble (pH 7.0)

异质活性

Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount

储存温度

−20°C

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 55 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 8 | 香港与北京 ≥ 10
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