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转染试剂,X-tremeGENE™ 360 Transfection Reagent;for molecular biology [罗氏]

将DNA, siRNA, miRNA, CRISPR/RNP输送到许多细胞系的通用聚合物试剂;罗氏Roche转染试剂;Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
货号(SKU): 8724121001
增值税发票 √ 免费货运 [顺丰 联邦] √
3.443,00 kr

产品描述

General description

X-tremeGENE 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE 360 delivers high efficiency transfection in more cell types with low cytotoxicity.

X-tremeGENE™360转染试剂是一种创新试剂,可与DNA或RNA形成复合物,并将核酸有效地传递到动物或昆虫细胞中。通过改进的实用性,X-tremeGENE™360可在多种细胞类型中以低细胞毒性实现高效转染。

Application

X-tremeGENE 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.
• Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
• Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
• Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
• Suitable for transient and stable transfection.
• Functions well in the presence or absence of serum.

X-tremeGENE™360转染试剂是一种高效的转染试剂,不含动物来源的成分。
•设计用于转染广泛的真核细胞,许多不能被其他试剂很好转染的细胞系,以及难以转染的细胞系,例如K-562和HepG2。
•可以成功用于各种应用中,例如使用瞬时转染的细胞进行基因表达分析,产生稳定的细胞系,用于基因敲除研究的siRNA表达以及CRISPR基因编辑工具。
•转染足够数量的细胞后,产生最小的细胞毒性或形态变化,从而消除了添加转染复合物后更换培养基的需求。
•适用于瞬时和稳定的转染。
•在有血清或无血清的情况下,功能良好。

Features and Benefits

X-tremeGENE 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE 360 exhibits:
• Peak performance in a broad range of cell types
• Suitable for multiple applications
• Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

X-tremeGENE™360转染试剂是针对难以转染和常见细胞类型的高性能,多功能和可靠的解决方案。作为一种经过验证的可靠试剂,X-tremeGENE™360使研究人员在进行转染时避免猜测。此外,X-tremeGENE™360还具有:
•在各种类型的电池中达到最佳性能
•适用于多种应用
•质粒DNA,siRNA / miRNA和CRISPR / Cas9组分的高效递送

Quality

Each lot of X-tremeGENE 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

每批X-tremeGENE™360转染试剂均按照既定的质量程序进行了仔细测试,以确保产品始终按照规格运行。在质量测试过程中,使用X-tremeGENE™360转染试剂(比例为3:1μL/μgDNA)用报告基因载体DNA转染细胞。通过化学发光检测来监测报告基因的活性。使用标准曲线,每孔确定重组蛋白的总量,以符合规格要求。

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

供应80%乙醇,并通过0.2μm孔径的膜进行无菌过滤。不含任何人类或动物来源的成分。测试数量:使用标准程序,可以在1孔X-tremeGENE™360转染试剂中进行96孔板的多达10,000次转染。

Other Notes

For life science research only. Not for use in diagnostic procedures.

法律信息

X-tremeGENE is a trademark of Roche

性质

gradefor molecular biology
formliquid (aqueous solution)
packagingpkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)
mfr. no.Roche
shipped inwet ice
storage temp.−20°C


安全信息

RIDADR NONH for all modes of transport
WGK德国 WGK 3
闪点(华氏) Not applicable
闪点(摄氏) Not applicable
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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