转化酶来源于面包酵母(酿酒酵母)，Invertase Glycoprotein Standard；BioReagent, from Saccharomyces cerevisiae, for proteomics

Invertase Glycoprotein Standard has been used:

• to generate N-linked glycan library
• as a negative control to study the binding of lectins to high mannose structures
• for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

用于生产糖果食品和人造蜂蜜。

 

转化酶将蔗糖水解成葡萄糖和果糖，产生无色产物，与产生有色产物的酸水解不同。

 

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.