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超敏葡琼凝胶-75 [制备级],Superdex 75 Prep Grade [GE Cytiva]

别名:葡琼凝胶G75;葡琼凝胶75;Superdex G75;GE Healthcare Cytiva Superdex-75;琼葡糖凝胶;葡琼糖凝胶;(安)法玛西亚;Amersharm Pharmacia Biotech;17104402;17104401;17104404;17-1044-02;17-1044-01;17-1044-04

Superdex 75制备级凝胶过滤树脂提供了非常高的分离度,可用于肽、小蛋白的分离、抛光和产品制备。
货号(SKU): 17104402
MDL号: MFCD00166816
增值税发票 √ 免费货运 [顺丰 联邦] √
£1,236.50

产品描述

Superdex 75 prep grade size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of recombinant proteins.

Superdex是一种超敏反应凝胶过滤填料,颗粒均匀、刚性好、不收缩,有极高的分辨率。

Application

Superdex® 系列也称琼葡糖凝胶、葡琼糖凝胶、葡琼凝胶,是将葡聚糖凝胶基质的高选择性与交联琼脂糖凝胶的化学和物理稳定性相结合,可用于蛋白质层析、凝胶过滤层析、凝胶过滤介质、树脂和分离介质。现已被用于从食用菌猴头菇中纯化和表征一种新型漆酶。Superdex还被用于紫荆种子作为潜在人类肝细胞癌抑制剂的纯化和机理研究。针对尺寸排阻色谱法的高分辨率制备分离进行了优化,列出了球状蛋白和葡聚糖的推荐分馏范围。

Superdex 75 prep grade size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of recombinant proteins.

  • Size exclusion chromatography resin providing exceptional resolution with short run times and good recovery.
  • Superdex 75 prep grade is recommended for purification of recombinant proteins.
  • BioProcess resin supported for industrial applications and well-established in approved processes.

Superdex prep grade is a preparative size exclusion chromatography resin with a composite matrix of dextran and agarose. This matrix combines the excellent size exclusion chromatography properties of cross-linked dextran (Sephadex) with the physical and chemical stabilities of highly cross-linked agarose, to produce a separation resin with outstanding selectivity and high resolution. In addition, its low non-specific interaction permits high recovery of biological material. Together these properties make Superdex prep grade the first choice size exclusion chromatography resin for all applications from laboratory to process scale.

Superdex prep grade is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As a BioProcess resin, Superdex prep grade meets industrial demands with security of supply and comprehensive technical and regulatory support.

Superdex prep grade is available in three selectivities: Superdex 30 prep grade is designed for purification of molecules in the molecular weight range up to 10 000 MW, such as peptides, oligosaccharides and small proteins. Superdex 75 prep grade is recommended for the purification of recombinant proteins. (Fractionation range 3000–70000 Da). Superdex 200 prep grade is recommended for the purification of monoclonal antibodies. (Fractionation range 10 000–600 000 Da)

性质

ParameterSuperdex 75 Prep Grade
BioProcess resinYes 
Particle size, d50V1~34 µm 
MatrixComposite of cross-linked agarose and dextran 
Particle Size24 µm-44 µm 
pH stability, operational23–12 
pH stability, CIP1–14 
Fractionation range (Mr) (globular proteins)3 × 10³–7 × 10⁴ 
Fractionation (Mp) Dextrans5 x 10²-3 x 10⁴ 
Storage4 to 30°C, 0.2 M sodium acetate in 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile 
Certificate of AnalysisYes 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.


安全信息

RIDADR
NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55