Superdex 100 Prep Grade size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of recombinant proteins.
Superdex是一种超敏反应凝胶过滤填料,颗粒均匀、刚性好、不收缩,在90cm/h流速下有极高的分辨率,Superdex 100 Prep Grade的分离范围:4,000~120,000 Da。
Application
Superdex® 系列也称琼葡糖凝胶、葡琼糖凝胶、葡琼凝胶,是将葡聚糖凝胶基质的高选择性与交联琼脂糖凝胶的化学和物理稳定性相结合,可用于蛋白质层析、凝胶过滤层析、凝胶过滤介质、树脂和分离介质。现已被用于从食用菌猴头菇中纯化和表征一种新型漆酶。Superdex还被用于紫荆种子作为潜在人类肝细胞癌抑制剂的纯化和机理研究。针对尺寸排阻色谱法的高分辨率制备分离进行了优化,列出了球状蛋白和葡聚糖的推荐分馏范围。Superdex prep grade is a preparative size exclusion chromatography resin with a composite matrix of dextran and agarose. This matrix combines the excellent size exclusion chromatography properties of cross-linked dextran (Sephadex) with the physical and chemical stabilities of highly cross-linked agarose, to produce a separation resin with outstanding selectivity and high resolution. In addition, its low non-specific interaction permits high recovery of biological material. Together these properties make Superdex prep grade the first choice size exclusion chromatography resin for all applications from laboratory to process scale.
Superdex prep grade is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As a BioProcess resin, Superdex prep grade meets industrial demands with security of supply and comprehensive technical and regulatory support.