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谷胱甘肽琼脂糖凝胶4B [GST标签蛋白纯化]，Glutathione Sepharose 4B GST-tagged protein purification resin

图片 谷胱甘肽琼脂糖凝胶4B [GST标签蛋白纯化]，Glutathione Sepharose 4B GST-tagged protein purification resin
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Glutathione Sepharose 4B 是一种亲和层析树脂，用于批量纯化 GST 标记的蛋白质以及需要高结合能力时。17075605；17075604；17075601；17-0756-05；17-0756-04；17-0756-01
制造商品牌: 思拓凡 Cytiva , (安)法玛西亚 Amersham Pharmacia
货号（SKU）: 17075605
技术支持 √ 增值税发票 √ 免费送货 √ 质量保障 √
¥4,100.00

Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required.

Glutathione Sepharose 4B 是一种亲和层析树脂，用于批量纯化 GST 标记的蛋白质以及需要高结合能力时。

高容量、单步谷胱甘肽 S-转移酶 (GST) 标记蛋白纯化。
以预溶胀的散装（松散）树脂的形式提供。
对所有常用的水性缓冲液具有化学稳定性。
适用于批量 GST 标记蛋白纯化和放大。
用于 GST 下拉分析以研究蛋白质-蛋白质相互作用。
在已发表的研究中成功使用
 

用于研究 RNA 结合蛋白与脱腺苷酸酶复合物核心亚基相互作用的 GST 下拉测定
研究调节初级纤毛发生的蛋白质-蛋白质相互作用网络
确定输入蛋白 8 在真核翻译起始因子的核输入中的作用

  • High capacity, single-step glutathione S-transferase (GST) tagged protein purification.
  • Supplied as pre-swollen bulk (loose) resin.
  • Chemically stable with all commonly used aqueous buffers.
  • Suitable for batch GST tagged protein purification and scale-up.
  • Used in GST pull-down assays to study protein-protein interactions.

Successful use in published research

Glutathione Sepharose 4B can be packed into the following empty column types: C, Tricorn, XK, and HiScale.

Compatible empty columns

Convenient prepacked columns

Gluthatione Sepharose 4B resin is also available in the following formats:

  • GSTrap 4B columns: HiTrap convenient format that can be used with a syringe, a peristaltic pump, or a chromatography system
  • GST MultiTrap 4B: 96 well plate format for high throughput screening and small scale purification using a centrifuge or vacuum equipment
  • GST GraviTrap: Gravity flow columns for direct purification of both clarified and unclarified cell lysates
  • GST SpinTrap: Microcentrifuge based spin columns for rapid small-scale purification of GST-tagged proteins

Other Cytiva products for recombinant protein purification

Glutathione Sepharose 4B is optimized for high yield batch purification. Cytiva offers other Life Sciences solutions for purifying GST fusion proteins. Use Cytiva's product selector to find GST-tagged protein purification resins and prepacked columns that meet your needs.

 

ParameterGlutathione Sepharose 4B
Chromatography techniqueGST-tagged protein purification 
LigandGlutathione 
Particle size, d50V1~90 μm 
MatrixAgarose, 4% 
Particle Size45 µm-165 µm 
Ligand Coupling MethodEpoxy activation 
Binding Capacity/ml Chromatography Medium2> 25 mg horse liver GST/mL resin 
pH stability, operational34–13 
pH stability, CIP44–13 
Storage4 to 30°C, 20% Ethanol 
Chemical stabilityCommonly used aqueous buffers 
LicensingFor licensing information, see the Licensing Statements page in the About Us section. 
  • 1Median particle size of the cumulative volume distribution.
  • 2Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
  • 3pH range where resin can be operated without significant change in function.
  • 4pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
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