Designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants.
Protein G Mag Sepharose Xtra 磁珠设计用于从血清和细胞上清液中快速、小规模纯化和筛选单克隆和多克隆抗体。
高效小规模纯化和筛选来自不同物种的抗体； 27 μg 人 IgG/μl 磁珠。
高容量，> 27 μg 人 IgG/μL 培养基，可以高效捕获高产量和纯度的抗体。从每次纯化运行中可以获得大约 350 μg 纯化的人 IgG。
Protein G Mag Sepharose Xtra 有用于 20 次纯化的 2 × 1 mL 包装和用于 50 次纯化的 5 × 1 mL 包装。该产品基于 Sepharose 介质，并加入磁铁矿以提高磁响应时间。该配体在 G 蛋白中是天然的，对来自各种物种的 IgG 抗体的片段结晶 (Fc) 区域具有高亲和力。
与 MagRack 6 和 MagRack Maxi 兼容，这些分离工具用于处理微量离心管中的珠子。
Protein G Mag Sepharose 是 Mag Sepharose 平台中的另一款产品，设计用于使用免疫沉淀技术从细胞裂解液和生物液体中快速捕获和富集蛋白质样品。它还由用于免疫沉淀的优化配体制成，允许使用与特定蛋白质特异性结合的抗体从溶液中去除蛋白质抗原。
Protein G Mag Sepharose Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants.
- Efficient small-scale purification and screening of antibodies from various species; 27 μg human IgG/μl magnetic beads.
- High-capacity, > 27 μg human IgG/μL medium, allows for the efficient capture of the antibody with high-yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.
- Robust parallel screening of antibodies with high reproducibility.
- Visible and dense Sepharose based magnetic beads give increased simplicity for handling.
- Non-adherent beads eliminate smearing effects and aggregate formation.
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.
- Can be used without detergents.
A large number of samples can easily be screened in parallel through a robotic device to meet high-throughput demands, allowing for simple capture of antibodies from small or large sample volumes (low microliter to high milliliter scale).
Protein G Mag Sepharose Xtra is available in 2 × 1 mL pack for 20 purifications as well as 5 × 1 mL pack for 50 purifications. The product is based on Sepharose medium with magnetite incorporated to enhance magnetic response times. The ligand is native in Protein G, with high affinity for the Fragment Crystallizable (Fc) region of IgG antibodies from a variety of species.
Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.
Protein G Mag Sepharose is another product in the Mag Sepharose platform, designed for the rapid capture and enrichment of protein samples from cell lysates as well as biological fluids using immunoprecipitation technology. It is also made with an optimized ligand for immunoprecipitation, allowing the removal of a protein antigen from a solution using an antibody specifically binding to that particular protein.
|Parameter||Protein G Mag Sepharose Xtra|
|Application||Designed to simplify small-scale purification and screening of antibodies Purified antibody for research studies |
|Matrix||Paramagnetic, spherical, highly cross-linked agarose particles |
|Ligand||Protein G |
|Binding Capacity||< > 27 mg human IgG/ml gel |
|Operating Temp.||Room temperature |
|Storage conditions||4–8°C, 20% Ethanol, 10% medium slurry |
|Chromatography technique||Antibody affinity chromatography |
|Bead type||Mag Sepharose Magnetic Beads |
|Pack size||5 × 1 mL |