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蛋白G-Mag琼脂糖凝胶,Protein G Mag Sepharose

该磁珠旨在通过免疫沉淀或下拉应用简化目标蛋白质的富集。28944008;28951379
货号(SKU): 28951379
增值税发票 √ 免费送货 √ 订货电话:010-68689484
$103.61

Magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications.

Protein G Mag Sepharose 通过免疫沉淀技术、使用抗体从溶液中沉淀蛋白质抗原或下拉应用作为检测物理相互作用的体外技术简化了目标蛋白质的富集。

具有蛋白质 G 配体的可见且致密的基于 Sepharose 的磁珠,可轻松发现和收集结合的目标蛋白质
非粘附珠消除了涂抹效应和聚集体形成
可以在没有洗涤剂的情况下使用
以小体积或大体积样品(从低微升到高毫升)简单捕获目标蛋白
优化目标蛋白的富集/免疫沉淀能力——只需要少量抗体
具有针对电泳和 MS 分析优化的洗脱条件的灵活方案
Protein G Mag Sepharose 将富集方法与磁珠相结合,用于小规模样品制备。这些磁珠涂有琼脂糖珠状琼脂糖,由于其对 IgG 抗体的 Fc 区具有高亲和力,因此以蛋白 G 作为配体。

有两种方案可用于蛋白质富集:交联方案,通过氨基酸之间的化学键将蛋白质连接在一起,以及蛋白质相互作用的经典方案。两者都是针对电泳和质谱 (MS) 优化的简单洗脱条件。

与 MagRack 6 和 MagRack Maxi 分离工具兼容,用于处理微量离心管中的珠子。

Protein G Mag Sepharose simplifies enrichment of target proteins by immunoprecipitation techniques, protein antigen precipitation from solutions using antibodies, or pull-down applications as an in vitro technique to detect physical interactions.

  • Visible and dense Sepharose based magnetic beads with protein G ligand making it easy to spot and collect bound target protein
  • Non-adherent beads eliminate smearing effects and aggregate formation
  • Can be used without detergents
  • Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target proteins—only low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

Protein G Mag Sepharose combines enrichment methods with magnetic beads, for small-scale sample preparation. These magnetic beads are coated with Sepharose, a beaded-form of agarose, with protein G as the ligand due to its high affinity for the Fc region of IgG antibodies.

Two protocols are available for protein enrichment: the cross-link protocol, linking proteins together via chemical bonds between amino acids, and the classic protocol for protein interaction. Both are simple elution conditions optimized for electrophoresis and mass spectrometry (MS).

Compatible with MagRack 6 and MagRack Maxi separation tools for handling the beads in microcentrifuge tubes.

 

ParameterProtein G Mag Sepharose
ApplicationDesigned to simplify enrichment of target proteins by immunoprecipitation or pull-down applications Used for biomarker reseach 
MatrixParamagnetic, spherical, highly cross-linked agarose particles 
LigandProtein G 
Binding Capacity> 13 mg human IgG/ml medium 
Operating Temp.Room temperature 
Storage conditions4–8°C, 20% Ethanol, 20% medium slurry 
StabilityStable 
Pack size4 × 500 μl 
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16