蛋白A琼脂糖凝胶CL-4B抗体纯化树脂,Protein A Sepharose CL-4B antibody purification resin

蛋白A琼脂糖凝胶CL-4B抗体纯化树脂是一种值得信赖的、有据可查的亲和树脂,适用于免疫沉淀程序。 也可用于抗体纯化。17096303;17096302;17078001
货号(SKU): 17096302
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Protein A Sepharose CL-4B is a trusted, well-documented affinity resin for immunoprecipitation procedures. It can also be used for antibody purification.

Protein A Sepharose CL-4B 是一种值得信赖的、有据可查的亲和树脂,适用于免疫沉淀程序。 也可用于抗体纯化。

20 mg 人 IgG/mL 的高结合容量。
Protein A Sepharose CL-4B 树脂具有高特异性和高容量,无论最终目标是确定蛋白质质量、分析蛋白质相互作用还是评估翻译后修饰,都可以可靠地进行精确、可重复的免疫沉淀程序。

  • High binding capacity of 20 mg human IgG/mL.
  • High selectivity and low nonspecific adsorption.
  • Well suited for immunoprecipitation procedures.

Use in immunoprecipitation procedures

Protein A Sepharose CL-4B resin offers high specificity and capacity, making it dependable for precise, reproducible immunoprecipitation procedures – whether the end goal is determining protein quality, analyzing protein interactions, or assessing post-translational modifications.

Successful use in published research

This protein A resin is used in a variety of applications, including:

ParameterProtein A Sepharose CL-4B, 500 mL
Chromatography techniqueAntibody affinity chromatography 
LigandNative Protein A 
Particle size, d50V1~90 µm 
MatrixCross-linked agarose, 4% 
Particle Size45 µm-165 µm 
Ligand Coupling MethodCyanogen bromide activation 
Binding Capacity/ml Chromatography Medium~20 mg human lgG/mL resin 
Ligand density3 mg Protein A/ml medium 
pH stability, operational23–9 
pH stability, CIP32–10 
Flow velocity< 150 cm/h at 25°C, HR 16/10 columns, 5 cm bed height 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers, 6 M guanidine-HCl, 8 M urea 
Sanitization Conditions2% Hibitane/20% Ethanol or with 70% Ethanol 
Certificate of AnalysisYes 
Pack size500 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16