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葡萄糖激酶来源于人类肝脏 [己糖激酶],Glucokinase from human liver [rHK];recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

别名:肝葡萄糖激酶; Hexokinase, Hexokinase, pancreatic, Pancreatic glucokinase

CAS号: 9001-51-8 NACRES: NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP6046
CAS号: 9001-51-8
MDL号: MFCD00131321
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¥3,049.16

说明

一般描述

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
 

单位定义

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
 

外形

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
 
 

属性

生物来源

human pancreas

重组

expressed in E. coli

测定

≥80% (SDS-PAGE)

形式

liquid

分子量

53.1 kDa

包装

pkg of 10 μg

浓度

1 mg/mL

杂质

Endotoxin, tested

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

Gene Information

human ... GCK(2645)

 

安全信息

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

商品规格
属性名称属性值
储存温度 Storage temp.-70°C超低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20