sevenspikes.themes.common.filters
sevenspikes.themes.common.closemenu

葡聚糖凝胶,Sephadex G-25 Superfine [G25超细, GE Cytiva]

别名:葡聚糖凝胶G-25;葡聚糖凝胶G25;(安)法玛西亚;Amersharm Pharmacia Biotech;17003101;17003102;17003103;17-0031-01;17-0031-02;17-0031-03

CAS号 9041-35-4 MDL number MFCD00132770
货号(SKU): 17003101
CAS号: 9041-35-4
MDL号: MFCD00132770
增值税发票 √ 免费货运 [顺丰 联邦] √
$289.53

产品描述

葡聚糖凝胶,即Sephadex,是一种凝胶过滤树脂,一种具有三维网状结构的高分子化合物,由葡聚糖加入交联剂通过醚键互相交联聚合而成。不同类型的Sephadex的交联度不同,因此其溶胀度和分子分级范围也不同。交联度越大,网孔结构就越紧密,吸水后的体积膨胀就越小,能允许进入凝胶内部的分子的分子量也就越小,反之亦然。层析时,大于凝胶孔径的大分子,被阻于凝胶相外,沿着凝胶颗粒之间的间隙流动,下移速度最快,故最先被洗脱下来;中分子物质进入凝胶内部,洗脱速度为其次;而小分子物质因全部进入凝胶,所受阻力最大,故最后被洗脱下来,藉此原理物质得到分离。

General description

Sephadex G-25 is well established gel filtration resin for desalting and buffer exchange in industrial applications

Application

超细葡聚糖凝胶® G-25是一种经常被引用的凝胶过滤介质,用于脱盐和缓冲液交换。葡聚糖凝胶® 是由葡聚糖与环氧氯丙烷交联而成。葡聚糖凝胶的交联程度不同,因此其溶胀程度和分子分馏范围也不同。“超细”表示可用的最小粒度。

超细葡聚糖凝胶® G-25是一种经常被引用的凝胶过滤介质,用于脱盐和缓冲液交换。葡聚糖凝胶 ® 是由葡聚糖与环氧氯丙烷交联而成。葡聚糖凝胶的交联程度不同,因此其溶胀程度和分子分馏范围也不同。“超细”表示可用的最小粒度。

葡聚糖凝胶® G-25 适用于缓冲液交换和脱盐寡核苷酸,用于测序、PCR 和标记应用。它用于分离从阿拉斯加鳕鱼皮提取的链霉蛋白酶 E 的两个不同的肽。

Features and Benefits

• 极佳的回收率
• 最小样品稀释度
• 快速且易于使用

性质

Sephadex G-25 is well established gel filtration resin for desalting and buffer exchange in industrial applications

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked HiTrap Desalting columns for fast and convenient desalting
  • BioProcess resin supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex G-25 is available in different bulk pack sizes and convenient pre-packed formats. As a BioProcess resin, Sephadex G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

ParameterSephadex G-25 Superfine
BioProcess resinYes 
MatrixCross-linked dextran 
Particle size distribution, wet15–88 µm 
Particle Size Dry>20 µm 
pH stability, operational12–13 
pH stability, CIP22–13 
Pressure/Flow Specification>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d. 
Fractionation range (Mr) (globular proteins)1 × 10³–5 × 10³ 
Fractionation (Mp) Dextrans1 x 10²-1 x 10² 
Exclusion limit (Mr) (globular proteins)~5 × 10³ 
Storage4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH 
Chemical stabilityCommonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile 
  • 1pH range where resin can be operated without significant change in function.
  • 2pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.


安全信息

RIDADR
NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
商品标签