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葡聚糖凝胶,Sephadex G-25 Fine [G25细颗粒, GE Cytiva]

别名:葡聚糖凝胶G-25;葡聚糖凝胶G25;(安)法玛西亚;Amersharm Pharmacia Biotech;17003201;17003202;17003203;17-0032-01;17-0032-02;17-0032-03

CAS号 9041-35-4 MDL number MFCD00132770
货号(SKU): 17003201
CAS号: 9041-35-4
MDL号: MFCD00132770
增值税发票 √ 免费货运 [顺丰 联邦] √
$227.79

产品描述

葡聚糖凝胶,即Sephadex,是一种凝胶过滤树脂,一种具有三维网状结构的高分子化合物,由葡聚糖加入交联剂通过醚键互相交联聚合而成。不同类型的Sephadex的交联度不同,因此其溶胀度和分子分级范围也不同。交联度越大,网孔结构就越紧密,吸水后的体积膨胀就越小,能允许进入凝胶内部的分子的分子量也就越小,反之亦然。层析时,大于凝胶孔径的大分子,被阻于凝胶相外,沿着凝胶颗粒之间的间隙流动,下移速度最快,故最先被洗脱下来;中分子物质进入凝胶内部,洗脱速度为其次;而小分子物质因全部进入凝胶,所受阻力最大,故最后被洗脱下来,藉此原理物质得到分离。

General description

Sephadex G-25 is well established gel filtration resin for desalting and buffer exchange in industrial applications

Application

Sephadex is a beaded gel filtration medium prepared by cross-linking dextran with epichlorohydrin under alkaline conditions.

Sephadex® G-25 is a gel filtration medium used in affinity chromatography, protein chromatography and gel filtration chromatography. Sephadex® G-25 has been used to study the acceleration of diabetic wound healing and the treatment of epithelial damage.

Sephadex® G-25 has been used in size-exclusion chromatography to remove excess dye from labelled proteins.

性质

Sephadex G-25 is well established gel filtration resin for desalting and buffer exchange in industrial applications

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked HiPrep Desalting columns for fast and convenient desalting
  • BioProcess resin supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine).

Sephadex G-25 is available in different bulk pack sizes and convenient pre-packed formats. As a BioProcess resin, Sephadex G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

ParameterSephadex G-25 Fine
BioProcess resinYes 
MatrixCross-linked dextran 
pH stability, operational12–13 
pH stability, CIP22–13 
Pressure/Flow Specification>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d. 
Fractionation range (Mr) (globular proteins)1 × 10³–5 × 10³ 
Fractionation (Mp) Dextrans1 x 10²-1 x 10² 
Exclusion limit (Mr) (globular proteins)~5 × 10³ 
Storage4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH 
Chemical stabilityCommonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol 
Certificate of AnalysisYes 
Swelling factor~1.7 
Particle size distribution, dry320 – 80 µm 
  • 1pH range where resin can be operated without significant change in function.
  • 2pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 3≥80% volume share within given range.


安全信息

RIDADR
NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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