Sephadex 为以下机构的注册商标： GE Healthcare
交联葡聚糖凝胶® G-100 是一种凝胶过滤介质，用于蛋白质色谱、亲和色谱和凝胶过滤色谱。
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Classic gel filtration resin.
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and resin are are preferred for large scale group separations where high flow rates and low operating pressures are required.
|Parameter||Sephadex G-100 Superfine|
|Matrix||Cross-linked dextran |
|Particle size distribution, wet||25–100 µm |
|Particle Size Dry||10 µm-40 µm |
|pH stability, operational1||2–10 |
|Flow rate||Care must be taken to avoid compressing the gel. Don not increase the pressure beyond 0.096 bar |
|Pressure/Flow Specification||Care must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar. |
|Fractionation range (Mr) (globular proteins)||4 × 10³ – 1 × 10⁵ |
|Fractionation (Mp) Dextrans||1 x 10³-1 x 10⁵ |
|Exclusion Limit||> 1 x 10⁵ |
|Autoclavable||In wet form (pH 7.0) at 120°C for 30 min |