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葡聚糖凝胶,Sephadex LH-20 [LH20, GE Healthcare Cytiva]

别名:亲脂交联葡聚糖凝胶LH20;Lipophilic Sephadex;羟基丙酸酯交联葡聚糖;赛哈德克斯;Smartdex LH20100;(安)法玛西亚;Amersharm Pharmacia Biotech;17009001;17009002;17009003;17009010;17-0090-01;17-0090-02;17-0090-03;17-0090-10

Sephadex LH-20 树脂用于天然产品的预纯化,如类固醇、三苯丙酮、脂质和低分子量肽等。
货号(SKU): 17009001
增值税发票 √ 免费货运 [顺丰 联邦] √
$404.49

产品描述

Sephadex LH-20 resin is used for preparative purification of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides.

Application

Sephadex® LH-20用于凝胶过滤层析、蛋白质层析、凝胶过滤介质、树脂及分离介质。Sephadex® LH-20已可用于研究糖尿病,并确定从野生,商业和非商业栽培黑莓基因型的生物活性多酚含量的差异。有研究应用Sephadex® LH-20首次报道了从莲藕水提物中分离色氨酸的方法,并对其抗氧化活性进行了研究。

Sephadex LH-20 resin is used for preparative purification of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides.

  • Broad stability in most aqueous mixtures and polar organic solvents.
  • Powerful chromatographic selectivity from the matrix’s dual hydrophilic/lipophilic nature.
  • High chemical and physical robustness.
  • High reproducibility from batch to batch.
  • Sephadex LH-20 is a resin specifically designed for molecular sizing of natural products in organic solvents.

Sephadex LH-20树脂用于天然产物的制备纯化,例如类固醇,类萜,脂质和低分子量肽。

在大多数水性混合物和极性有机溶剂中具有广泛的稳定性。
基质具有双重亲水/亲脂特性,因此具有强大的色谱选择性。
高化学和物理强度。
批次之间的重复性高。
Sephadex LH-20是专为有机溶剂中天然产物的分子筛分设计的树脂。

Sephadex LH-20 resin for organic solvent use

This chromatography resin is made from cross-linked Sephadex G-25 dextran beads, hydroxypropylated to yield both hydrophilic and lipophilic characteristics.

This resin is useful for molecular species preparation at different scales. It can be used during purification, prior to high performance ion exchange or reversed phase chromatography (RPC), or as a final polishing step during diastereomer preparation.

Sephadex LH-20有机溶剂用树脂
该色谱树脂由交联的Sephadex G-25葡聚糖珠制成,羟丙基化以产生亲水和亲脂特性。

该树脂可用于不同规模的分子物种制备。它可以在纯化过程中,高性能离子交换或反相色谱法(RPC)之前使用,或在非对映体制备过程中用作最终的抛光步骤。

Successful use in published research

Applications of Sephadex LH-20 resin include lipid fractionation and purifying complex organic substances, such as traditional Chinese medicine (TCM), for research use. Examples of successful research use include:

成功用于已发表的研究

Sephadex LH-20树脂的应用包括脂质分级分离和纯化复杂的有机物质,例如中药(TCM)供研究使用。成功使用研究的例子包括:

相思树茎皮中的抗氧化活性;
玉米花粉和apis mellifera蜂箱中发现的花粉中的自由基清除活性;
松树针叶中的抗氧化活性;

Sephadex 为以下机构的注册商标: GE Healthcare

性质

ParameterSephadex LH-20
MatrixHydroxypropylated, cross-linked dextran 
Particle Size Dry18 µm-111 µm 
Particle Size in Methanol Min.27 µm
Particle Size in Methanol Max.163 µm
Sample Loading VolumeDetermined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode] 
ApplicationFor fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures 
pH stability, operational12–13 
pH stability, CIP22–13 
Pressure/Flow Specification25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d. 
Flow velocity< 60 cm/hour 
Fractionation range (Mr) (globular proteins)4 × 10³ (E×clusion limit will depend on the solvent)–5 × 10³ (E×clusion limit will depend on the solvent) 
Exclusion Limit4 x 10³ (for Peptides) 5 x 10³ (small organics) 
Exclusion Limit Peptides4 x 10³ 
Exclusion Limit Small Organics5 x 10³ 
Storage4 to 30°C 
Estimated shelf life from manufacture date8 years 
Chemical stabilityStable in most aqueous and organic eluent systems. 
Avoid UsingNot stable below pH 2 nor to strong oxidising agents 
Autoclavable20 min at 121°C 
Pack size100 g 
  • 1pH range where resin can be operated without significant change in function.
  • 2pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.


安全信息

RIDADR
NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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