Sephadex LH-20 resin for organic solvents
Sephadex LH-20 resin is used for preparative purification of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides.
在大多数水性混合物和极性有机溶剂中具有广泛的稳定性。
来自基质的双重亲水/亲油性质的强大色谱选择性。
高化学和物理稳定性。
批次间的高重现性。
Sephadex LH-20 是一种专门设计用于有机溶剂中天然产物分子筛分的树脂。
有机溶剂用Sephadex LH-20树脂
这种层析树脂由交联的 Sephadex G-25 葡聚糖珠制成，经羟丙基化后具有亲水性和亲脂性。
这种树脂可用于不同规模的分子种类制备。它可用于纯化过程中、高效离子交换或反相色谱 (RPC) 之前，或作为非对映异构体制备过程中的最终精制步骤。
在已发表的研究中成功使用
Sephadex LH-20 树脂的应用包括脂质分馏和纯化复杂的有机物质，如中药 (TCM)，用于研究用途。成功的研究用途的例子包括：
金合欢茎皮的抗氧化活性
花玉米花粉和蜜蜂蜂巢中发现的花粉中的自由基清除活性
红松针叶的抗氧化活性
- Broad stability in most aqueous mixtures and polar organic solvents.
- Powerful chromatographic selectivity from the matrix’s dual hydrophilic/lipophilic nature.
- High chemical and physical robustness.
- High reproducibility from batch to batch.
- Sephadex LH-20 is a resin specifically designed for molecular sizing of natural products in organic solvents.
Sephadex LH-20 resin for organic solvent use
This chromatography resin is made from cross-linked Sephadex G-25 dextran beads, hydroxypropylated to yield both hydrophilic and lipophilic characteristics.
This resin is useful for molecular species preparation at different scales. It can be used during purification, prior to high performance ion exchange or reversed phase chromatography (RPC), or as a final polishing step during diastereomer preparation.
Successful use in published research
Applications of Sephadex LH-20 resin include lipid fractionation and purifying complex organic substances, such as traditional Chinese medicine (TCM), for research use. Examples of successful research use include:
| Parameter | Sephadex LH-20 |
|---|
| Matrix | Hydroxypropylated, cross-linked dextran |
| Particle Size Dry | 18 µm-111 µm |
| Particle Size in Methanol Min. | 27 µm |
| Particle Size in Methanol Max. | 163 µm |
| Sample Loading Volume | Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode] |
| Application | For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures |
| pH stability, operational1 | 2–13 |
| pH stability, CIP2 | 2–13 |
| Pressure/Flow Specification | 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d. |
| Flow velocity | < 60 cm/hour |
| Fractionation range (Mr) (globular proteins) | 4 × 10³ (E×clusion limit will depend on the solvent)–5 × 10³ (E×clusion limit will depend on the solvent) |
| Exclusion Limit | 4 x 10³ (for Peptides) 5 x 10³ (small organics) |
| Exclusion Limit Peptides | 4 x 10³ |
| Exclusion Limit Small Organics | 5 x 10³ |
| Storage | 4 to 30°C |
| Estimated shelf life from manufacture date： | 8 years |
| Chemical stability | Stable in most aqueous and organic eluent systems. |
| Avoid Using | Not stable below pH 2 nor to strong oxidising agents |
| Autoclavable | 20 min at 121°C |
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