葡聚糖凝胶G15,Sephadex G-15

Sephadex G-15 是一种成熟的凝胶过滤树脂,用于肽和分子量大于 1500 的小生物分子的脱盐和缓冲液交换。17002003;17002001
货号(SKU): 17002001
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Sephadex G-15 is well established gel filtration resin for desalting and buffer exchange of peptides and small biomolecules >1 500 molecular weight.

适用于生物样品的缓冲液交换和净化,例如分子量大于 1 500 的肽、小蛋白和寡糖
Sephadex 是一种凝胶过滤树脂,由葡聚糖与环氧氯丙烷交联而成。 不同类型的 Sephadex 的交联度不同,因此溶胀度和分子分级范围也不同。 Sephadex G-15 是五种不同的 G 型之一,范围从用于小分子的 G-10 到用于较大分子的 G-75。 Sephadex G-50 有 4 种不同的粒径(粗、树脂、细和超细),而超细具有最小的珠粒尺寸,可在更短的扩散距离下获得更高的效率。 对于需要高流速和低操作压力的大规模组分离,粗颗粒和树脂是首选。

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 1 500 molecular weight
  • Efficient removal of contaminants such as salts, dyes, and radioactive labels

Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and resin are are preferred for large scale group separations at where high flow rates and low operating pressures are required.


ParameterSephadex G-15, 100 g
MatrixCross-linked dextran 
Particle size distribution, wet60–180 µm 
Particle Size Dry40 µm-120 µm 
pH stability, operational12–13 
Flow rate2< Darcy´s Law 
Pressure/Flow Specification20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d. 
Fractionation range (Mr) (globular proteins)<1.5 × 10³ 
Fractionation (Mp) Dextrans>< 1.5 x 10³ 
Storage4 to 30°C 
AutoclavableIn wet form (pH 7.0) at 120°C for 30 min 
Pack size100 g 
  • 1pH range where resin can be operated without significant change in function.
  • 2The beads behave as rigid spheres obeying Darcy's Law.
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32