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低取代苯基琼脂糖凝胶6FF,Phenyl Sepharose 6 Fast Flow (Low Sub)

苯基琼脂糖凝胶6FF是一种成熟的标准芳香族疏水相互作用色谱 (HIC) 树脂,用于需要低至树脂疏水性的捕获和中间纯化。17096503;17096504;17096505;17096510
货号(SKU): 17096503
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 19,525.39

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

基于 Sepharose Fast Flow 基质的标准芳香族 HIC 树脂,通过不带电荷的、化学稳定的醚键衍生。
设计用于需要低至中等疏水性的初始和中间纯化
两个级别的替代有助于为给定的应用找到最佳的选择性和结合能力
BioProcess 树脂支持工业应用,并在批准的工艺中建立良好
基础基质的亲水性确保了低水平的非特异性结合。
Phenyl Sepharose 6 Fast Flow 是 Sepharose Fast Flow HIC 平台的一部分,该平台在近几十年来一直是 HIC 色谱的工业标准。 Phenyl Sepharose 6 Fast Flow 由交联的 6% 琼脂糖珠组成,通过不带电荷的、化学稳定的醚键用标准芳族苯基修饰。两个水平的配体取代度有助于找到给定应用的最佳选择性和结合能力。

Phenyl Sepharose 6 Fast Flow 有多种不同的散装尺寸和方便的预包装形式,便于放大和工艺开发。作为 BioProcess 树脂系列的一员,Phenyl Sepharose 6 Fast Flow 通过供应安全和全面的技术和法规支持满足工业需求

 

  • Standard aromatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Designed for initial and intermediate purification requiring low to medium hydrophobicity
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Phenyl Sepharose 6 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Phenyl Sepharose 6 Fast Flow is composed of cross-linked, 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.

Phenyl Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Phenyl Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

 

ParameterPhenyl Sepharose 6 Fast Flow (Low Sub)
BioProcess resinYes 
LigandPhenyl 
Particle size, d50V1~90 µm 
Matrixcross-linked agarose, 6%, spherical 
Particle Size45 µm-165 µm 
Ligand Concentration~25 µmol phenyl/mL resin 
pH stability, operational23–13 
pH stability, CIP32–14 
Pressure/flow characteristics4250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water). 
Storage4 to 30°C, 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea 
Certificate of AnalysisYes 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 4The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16