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脱氧核糖核酸酶I来源于牛胰腺,Deoxyribonuclease I from bovine pancreas [DNase I];lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein

别名:DNA酶I;脱氧核糖核酸 5′-寡核苷酸-水解酶;脱氧核糖核酸酶I来源于牛胰腺;Deoxyribonucleate 5′-oligonucleotido-hydrolase

CAS号 9003-98-9 Enzyme Commission (EC) Number 3.1.21.1 ( BRENDA | IUBMB ) EINECS 编号 232-667-0 MDL number MFCD00130918
货号(SKU): DN25
CAS号: 9003-98-9
MDL号: MFCD00130918
增值税发票 √ 免费货运 [顺丰 联邦] √
¥550.16

产品描述

General description

DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
• Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
• Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
• DNase I structure resembles the structure of to exonuclease III. It includes two central ß sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]

Application

• Decreases viscosity providing better yields by removing DNA in primary cell isolation:
• Incorporating labelled bases into DNA: DNA nick
• Radioactive labelling
• Bioprocessing applications: DNA removal
• Eliminating genomic DNA from RNA preparations before RT-PCR
• In vitro transcription
• Nick translation
• DNase footprinting
• Actin regulation of actin polymerization in cells, and cell apoptosis
• UV crosslinking of proteins to nucleic acids
• DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process [1]

用于从蛋白质样品中除去 DNA。

包装

1, 5 g in poly bottle

10, 100 mg in poly bottle

Biochem/physiol Actions

DNase I是一种内切核酸酶,可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg2+存在时,DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn2+存在时,两条DNA链都几乎是在同样的位置被切割。[6]二价阳离子如Mn2+, Ca2+, Co2+以及Zn2+都是酶的激活剂。5 mM浓度的Ca2+可稳定酶免收蛋白水解酶的消化。最适pH在7-8之间[7]来自牛胰腺的DNAse I由四种色谱可区分的组分A,B,C和D组成,它们的摩尔比分别为4:1:1。仅发现少量D。[5]2-巯基乙醇、螯合剂、十二烷基硫酸钠(SDS)[4]和肌动蛋白[1]都已知可抑制酶的活性。

Features and Benefits

Our Deoxyribonuclease DNAse I,is essentially RNAse free product, which support the product application.

Unit Definition

一Kunitz单位的酶在以I型或III型DNA作为底物时,在 pH 5.0,37°C的条件下在每mL中每分钟可引起0.001的A260改变。

Physical form

粗制品,含有氯化钙

Preparation Note

溶液0.15 M NaCl的10 mg/mL DNAse I溶液分装保存在−20 °C一周后可能会丢失<10%的活性。同样的溶液保存在2-8 °C则可能丢失约20%的活性。当在pH 5和7之间时,它可在60 °C维持活性最长达五小时,并在68 °C <10分钟内丢失活性。它在乙酸缓冲液(pH 5.0)和tris缓冲液((pH 7.2),1 mg/mL浓度)中以6%/小时的速率丢失活性。

Analysis Note

Protein determined by biuret.

性质

Quality Level300
formlyophilized powder
specific activity≥400 Kunitz units/mg protein
mol wt~31 kDa
compositionProtein, ≥85%
solubility0.15 M NaCl: soluble 5.0 mg/mL, hazy
Featured IndustryDiagnostic Assay Manufacturing
Diagnostic Assay Manufacturing
foreign activityRNase ≤0.02%
shipped inwet ice
storage temp.−20°C


安全信息

符号
GHS08  GHS08
信号词 Danger
危害声明 H334
个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
RTECS RF0750000
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 88 | 欧洲Eur. ≥ 126 | 東京Tokyo ≥ 46 | 香港与北京 ≥ 62
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