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肠激酶来源于猪肠 [肠肽酶],Enterokinase from porcine intestine;lyophilized powder, ≥100 units/mg protein

别名:猪肠激酶;Enteropeptidase;CAS号 9014-74-8 Enzyme Commission (EC) Number 3.4.21.9 ( BRENDA | IUBMB ) EINECS 编号 232-761-1 MDL number MFCD00131020 eCl@ss 32160410 NACRES NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): E0885
CAS号: 9014-74-8
MDL号: MFCD00131020
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¥2,461.57

产品描述

Application

Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide.

The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen.[1] The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus[2] The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa.[3] It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.[4]

Biochem/physiol Actions

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

Unit Definition

在 pH 5.6 和 25°C 下,一单位每分钟可将胰蛋白酶原转化为 1.0nmol 的胰蛋白酶。

Physical form

含有磷酸钠缓冲盐的冻干粉

性质

Quality Level200
formlyophilized powder
specific activity≥100 units/mg protein
mol wt150 kDa
purified bychromatography
compositionProtein, ≥20% Lowry
foreign activityaminopeptidase ≤1.5%
trypsin ≤1%, free
storage temp.−20°C


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16