罗丹明123，Rhodamine 123；BioReagent, for fluorescence, ≥85% (HPLC)

General description

Rhodamine 123 is a fluorescent dye.^[3]

Application

Rhodamine 123 has been used:
• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays^[2]
• to label the cells for estimating the P-gp expression by flow cytometry^[3]
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)^[4]
• the detection of mitochondrial membrane potential (ΔΨm)^[5]

Biochem/physiol Actions

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.^[3]

最常用于流式细胞术的荧光染料，可用作Pgp的功能性报告分子。在癌症治疗中，MDR蛋白的功能测定是比MDR蛋白表达水平更好的预后指标。Rh 123可用于多参数分析而无荧光干扰，可与常用蛋白质标记染料组合使用，如PE-Cy5和AMCA（7-氨基-4-甲基香豆素-3-乙酸）。近期报道表明，Rh123也可能是MRP1的底物。

Other Notes

上活体线粒体特异性染料^[1]

性质

安全信息