General description
Rhodamine 123 is a fluorescent dye.[3]
Application
Rhodamine 123 has been used:
• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays[2]
• to label the cells for estimating the P-gp expression by flow cytometry[3]
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)[4]
• the detection of mitochondrial membrane potential (ΔΨm)[5]
Biochem/physiol Actions
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.[3]
最常用于流式细胞术的荧光染料,可用作Pgp的功能性报告分子。在癌症治疗中,MDR蛋白的功能测定是比MDR蛋白表达水平更好的预后指标。Rh 123可用于多参数分析而无荧光干扰,可与常用蛋白质标记染料组合使用,如PE-Cy5和AMCA(7-氨基-4-甲基香豆素-3-乙酸)。近期报道表明,Rh123也可能是MRP1的底物。Other Notes
上活体线粒体特异性染料[1]