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磷酸酶(碱性)来源于人类胎盘,Phosphatase, Alkaline from human placenta;≥10 units/mg solid (in glycine buffer)

别名:人碱性磷酸酶;Phosphatase, Alkaline from human placenta, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

CAS号: 9001-78-9 EC 号: 3.1.3.1 (BRENDA, IUBMB) MDL编号: MFCD00131849
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SAE0111
CAS号: 9001-78-9
MDL号: MFCD00131849
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¥1,445.74

说明

应用

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.
 

生化/生理作用

Alkaline phosphatase dephosphorylates proteins and nucleic acids. Human placental alkaline phosphatase (PLAP) has an unique residue, Glu-429. It is an allosteric enzyme that is uncompetitively inhibited by L-Phe, L-Trp, and L-Leu. PLAP is post-translationally modified. Phosphatidylinositol-specific phospholipase C may be involved in the release of PLAP. PLAP may have a role in cell division and may be involved in the transfer of maternal IgG to the fetus.
 

单位定义

One unit will hydrolyze 1.0 umole of P-Nitrophenyl Phosphate per minute at pH 10.4 at 37 Deg C.
 
 

属性

生物来源

human placenta

specific activity

≥10 units/mg solid (in glycine buffer)

UniProt登记号

储存温度

−20°C

Gene Information

human ... ALPP(250)

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20
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