General description
Concanavalin A is isolated from the from Canavalia ensiformis (Jack bean). It is a mitogenic lectin with a dome-like structures and is a tetrameric protein.[1]
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
• in the colabelling of fischer rat thyroid (FRT) epithelia(104)
• to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105)
• to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)
Biochem/physiol Actions
Concanavalin A (Con A) bound to glycoenzyme has wide applications and can be dissociated in the presence of high sugar levels.[3] Con A induces hepatic inflammation.[4] It stimulates macrophages and promotes cytokine production. Con A also exhibits anti-hepatoma effect and could be used effectively as a vaccine for treating cancer.[2]
刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。包装
基于蛋白含量进行包装
Physical form
含有柠檬酸钠
Analysis Note
在报道中,凝集活性以μg/ml表示,并且是在含有1mg/mL Ca 2+和Mn 2+的磷酸盐缓冲盐水(pH 6.8)的系列稀释液中进行测定的。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。