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甘油醛-3-磷酸脱氢酶来源于人 [GAPDH],Glyceraldehyde-3-phosphate dehydrogenase human;recombinant, expressed in Escherichia coli, ≥80 units/mg protein

别名:D-甘油醛-3-磷酸:NAD+ 氧化还原酶(磷酸化); D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

CAS号 9001-50-7 Enzyme Commission (EC) Number 1.2.1.12 ( BRENDA | IUBMB ) MDL number MFCD00131203 NACRES NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRE0024
CAS号: 9001-50-7
MDL号: MFCD00131203
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产品描述

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) localizes to the cytoplasm but can be translocated to the nucleus depending on cellular conditions. It is a tetramer containing identical chains. The gene encoding GAPDH is localized on human chromosome 12p13.

Application

Human glyceraldehyde-3-phosphate dehydrogenase has been used as a positive control for hydrogen sulfide treatment to detect sulfhydration of protein targets.[8]

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy) and huntingtin. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway.[3] GAPDH is also a component of the functional GAIT (interferon-?-activated inhibitor of translation) mRNP (messenger ribonucleoprotein).[5] GAPDH expression is dysregulated during melanoma progression.[7]

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per minute in a coupled system with 3-phosphoglyceric phosphokinase (3-PGK) at pH 7.6 at 25 °C

Physical form

This product is recombinant, human GAPDH expressed in E. coli with an N-terminal histidine tag and has a predicted molecular mass of 37,984 Da. The product is lyophilized from a buffered solution with stabilizers.

Other Notes

Alternative number: CAS Number 9001-50-7

性质

Quality Level200
recombinantexpressed in E. coli
specific activity≥80 units/mg protein
storage temp.−20°C


安全信息

RIDADR NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
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