溴酚蓝，Bromophenol Blue [BPB]；powder

图片 溴酚蓝，Bromophenol Blue [BPB]；powder
别名：溴酚兰；3′,3′′,5′,5′′-四溴酚磺酰酞；四溴酚磺酞；溴酚蓝磺酸内酯；溴酚蓝（BPB）； 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form

Empirical Formula (Hill Notation): C19H10Br4O5S CAS号: 115-39-9 分子量: 669.96 Beilstein: 61698 EC 号: 204-086-2 MDL编号: MFCD00005875 PubChem化学物质编号: 24891504 NACRES: NA.47
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: B0126
CAS号: 115-39-9
MDL号: MFCD00005875
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¥533.34

说明

溴酚蓝作为磺化羟基醌存在于酸性水溶液中。它也被称为四溴苯酚磺酞。溴酚蓝是pH指示剂，染料呈现强烈的蓝色。它具有轻微的负电荷，并将以与DNA相同的方向迁移，使用户可以监测通过凝胶移动的分子的进展。迁移速率随凝胶组成而变化。
溴酚蓝用作DNA，RNA（琼脂糖）和蛋白质（聚丙烯酰胺）凝胶电泳中的跟踪染料。它已被用于蛋白质样品的制备用于蛋白质印迹分析。溴酚蓝在琼脂糖凝胶和蛋白质聚丙烯酰胺凝胶中的缓冲液前沿以与300-500bp DNA大致相同的速率迁移。它还被称为用作探测血脑屏障的重要染色剂。

一般描述

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
 

应用

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.
 
 

属性

形式

powder

质量水平

200

technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

溶解性

methanol: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20
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