溴化氢活化琼脂糖凝胶4FF,CNBr-Activated Sepharose 4 Fast Flow

CNBr 活化的 Sepharose 4 Fast Flow 是一种成熟的预活化层析树脂,用于偶联大的含氨基配体。17098105;17098101;17098103
货号(SKU): 17098103
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CNBr-activated Sepharose 4 Fast Flow is a well established, pre-activated chromatography resin for coupling of large amino-containing ligands.

CNBr 活化的 BioProcess 树脂设计用于偶联大的含氨基配体。
BioProcess 树脂支持工业应用,并在批准的工艺中建立良好
CNBr 活化的 Sepharose 4 Fast Flow 基于完善的 Sepharose Fast Flow 平台。该树脂由已用溴化氰预活化的交联 4% 琼脂糖珠组成。 CNBr 活化的 Speharose 4 Fast Flow 设计用于多点连接含有氨基的蛋白质配体。

通过将生物特异性配体与 CNBr 活化的 Sepharose 4 Fast Flow 偶联来制备和使用亲和层析树脂是一种广泛使用、成功且有据可查的方法,具有简单、快速和高效的偶联程序。

CNBr 活化的 Sepharose 4 Fast Flow 有多种不同的散装尺寸和方便的预包装形式,便于放大和工艺开发。作为 BioProcess 树脂系列的一员,CNBr 活化的 Sepharose 4 Fast Flow 满足工业需求,具有供应安全性和全面的技术和法规支持

  • CNBr-activated BioProcess resin designed for coupling of large amino-containing ligands.
  • Easy, rapid and efficient coupling
  • This resin allows for multi-point attachment of protein ligands which minimizes ligand leakage
  • BioProcess resin supported for industrial applications and well-established in approved processes

CNBr-activated Sepharose 4 Fast Flow is based on the well established Sepharose Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Speharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups.

The preparation and use of affinity chromatography resins by coupling biospecific ligands to CNBr-activated Sepharose 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.

CNBr-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, CNBr-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support


ParameterCNBr-Activated Sepharose 4 Fast Flow, 250 g
Chromatography techniqueAffinity coupling 
BioProcess resinYes 
LigandCyanogen bromide activated 
Particle size, d50V1~90 µm 
Group To be CoupledPrimary amines 
Matrix4% cross-linked agarose 
Particle Size45 µm-165 µm 
pH stability, operational23–11 
pH stability, CIP2–11 (ligand dependent) 
Pressure/Flow SpecificationBase matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm 
StorageUnsused: 2 to 8°C - Used: 2 to 8°C in 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment 
Certificate of AnalysisYes 
Pack size250 g 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32