氯霉素，Chloramphenicol；VETRANAL®, analytical standard, ≥98% (HPLC)

General description

Chloramphenicol (CAP) is an antibiotic which was first isolated from Streptomyces venezuelae. It has a nitrobenzene moiety which may be responsible for aplastic anaemia.1 It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.
This grade has the standard for Supelco MIP SPE cartridges. For more information request Supelco Literature T407075, T706024

Application

Chloramphenicol has been used as reference standard in the determination of the concentration of CAP residues in shrimp tissues using LC-MS technique^[1] and also in frozen chicken samples (liver, kidney and muscle) using HPLC.^[2]

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Biochem/physiol Actions

Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.
Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).

Caution

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

Preparation Note

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

法律信息

VETRANAL is a registered trademark of Sigma-Aldrich Chemie GmbH

性质

安全信息

符号

  GHS08

信号词 Warning

危害声明 H351

警示性声明 P201 - P308 + P313

个人防护装备 Eyeshields, Faceshields, Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

危害码 (欧洲) T

风险声明 (欧洲) 45

安全声明 (欧洲) 53-45

RIDADR NONH for all modes of transport

WGK德国 WGK 3

RTECS AB6825000