氯霉素，Chloramphenicol；VETRANAL®, analytical standard, ≥98% (HPLC)

图片 氯霉素，Chloramphenicol；VETRANAL®, analytical standard, ≥98% (HPLC)
别名：氯霉素标准品；D-苏式-(-)-N-[α-(羟基甲基)-β-羟基-对硝基苯乙基]-2,2-二氯乙酰胺；(1R,2R)-N-[α-(羟基甲基)-β-羟基-对硝基苯乙基]-2,2-二氯乙酰胺；D-(-)-苏-1-对硝基苯基-2-二氯乙酰胺基-1,3-丙二醇；左旋霉素；左旋-苏-1-(对硝基苯基)-2-二氯乙酰氨基-1,3-丙二醇；D(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide, Chloromycetin

线性分子式: Cl2CHCONHCH(CH2OH)CH(OH)C6H4NO2 CAS号: 56-75-7 分子量: 323.13 Beilstein: 2225532 EC 号: 200-287-4 MDL编号: MFCD00078159 PubChem化学物质编号: 329753874 NACRES: NA.24
制造商品牌: 色谱科 Supelco , 西格玛 Sigma-Aldrich
货号（SKU）: 31667
CAS号: 56-75-7
MDL号: MFCD00078159
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说明

氯霉素（CAP）是一种首先从委内瑞拉链霉菌中分离出来的抗生素。它具有可能导致再生障碍性贫血的硝基苯部分。1它通常用于10-20μg/mL的分子生物学应用中的细菌选择，并作为含有氯霉素抗性基因的转化细胞的选择剂。
氯霉素已被用作参考标准，用于使用LC-MS技术测定虾组织中CAP残留的浓度，以及使用HPLC测定冷冻鸡样品（肝脏，肾脏和肌肉）中的CAP残留浓度。

一般描述

Chloramphenicol (CAP) is an antibiotic which was first isolated from Streptomyces venezuelae. It has a nitrobenzene moiety which may be responsible for aplastic anaemia.1 It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.
This grade has the standard for Supelco MIP SPE cartridges. For more information request Supelco Literature T407075, T706024
 

应用

Chloramphenicol has been used as reference standard in the determination of the concentration of CAP residues in shrimp tissues using LC-MS technique and also in frozen chicken samples (liver, kidney and muscle) using HPLC.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
 

生化/生理作用

作用机制：氯霉素通过与50S核糖体亚基结合并阻止氨酰基tRNA附着于核糖体来阻断肽基转移酶步骤，从而抑制细菌蛋白质的合成。 它还抑制线粒体和叶绿体蛋白的合成以及（p）ppGpp的核糖体形成，从而抑制rRNA的转录。

耐药性机制：使用氯霉素乙酰转移酶会使产物乙酰化并使其失活。
抗菌谱：这是一种针对革兰氏阳性和革兰氏阴性细菌的广谱抗生素，主要用于眼科和兽医目的。
 

注意

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.
 

制备说明

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.
 

法律信息

VETRANAL is a registered trademark of Sigma-Aldrich Chemie GmbH
 
 

属性

等级

analytical standard

质量水平

100

产品线

VETRANAL®

测定

≥98% (HPLC)

保质期

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

148-150 °C (lit.)

溶解性

H2O: insoluble 100% (practically)

application(s)

clinical testing

格式

neat

SMILES string

OC[C@@H](NC(=O)C(Cl)Cl)[C@H](O)c1ccc(cc1)[N+]([O-])=O

InChI

1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1

InChI key

WIIZWVCIJKGZOK-RKDXNWHRSA-N

 

安全信息

象形图

CorrosionHealth hazard
GHS05,GHS08

警示用语：

Danger

危险分类

Carc. 2 - Eye Dam. 1 - Repr. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

商品规格
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储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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