General description
通过与50S核糖体亚基结合来抑制RNA到蛋白质的翻译,从而阻止肽基转移酶反应的合成抑菌抗生素。氯霉素抗性由抗性基因cat编码,其产物氯霉素乙酰转移酶可通过乙酰化使抗生素失活。通常以30-50 µg/ml用于筛选带有氯霉素抗性的大肠杆菌。通过阻断核糖体上肽基转移酶反应来抑制RNA翻译的合成抑菌抗生素。包装
100, 500 g in Glass bottle
25 g in Plastic ampoule
Biochem/physiol Actions
功效:≥970 µg/mg
首要靶标
RNA的翻译
Other Notes
Lech, K., and Brent, R. 1995. in Current Protocols in Mol.Biol. Vol. 1, (Ausubel, F.M., et al., Eds.)John Wiley & Sons, New York.
Lu, J., and Jiang, C. 1993.Biochem.Biophys.Res. Commun.196, 12.
Saltarelli, M.J., et al. 1993.Virol.197, 35.
Holt, J.T. 1992.Ann.N.Y.Acad.Sci.660, 88.
Maniatis, T., et al. 1989.In Molecular Cloning, A Laboratory Manual, Second Edition.Cold Spring Harbor, NY, p. 1.6, A.6.