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核糖核酸酶A来源于牛胰腺 [RNA酶A],Ribonuclease A from bovine pancreas [RNase A];Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

别名:RNaseA;核糖核酸 3′-嘧啶寡核苷酸水解酶;核糖核酸酶 I;胰核糖核酸酶;RNase-A;Pancreatic Ribonuclease;Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

CAS号 9001-99-4 Enzyme Commission (EC) Number 3.1.27.5 ( BRENDA | IUBMB ) EINECS 编号 232-646-6 MDL number MFCD00132181 NACRES NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): R5500
CAS号: 9001-99-4
MDL号: MFCD00132181
增值税发票 √ 免费货运 [顺丰 联邦] √
¥517.88

产品描述

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Application

• RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
• RNase A is also used in RNA sequence analysis and protection assays.
• RNase A has been used as a tool for computer-aided drug design.
• RNase A supports the analysis of RNA sequences.
• RNase A hydrolyze RNA contained in protein samples.
• Purification of DNA is supported by RNase A.

包装

1 g in glass bottle

100, 250, 500 mg in glass bottle

10 mg in poly bottle

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Preparation Note

Salt fractionated and chromatographically purified.

Analysis Note

Protein determined by E.

性质

Quality Level200
typeType XII-A
assay≥90% (SDS-PAGE)
formlyophilized powder
specific activity75-125 Kunitz units/mg protein
mol wt~13,700
impuritiessalt, essentially free
foreign activityprotease, essentially free
storage temp.−20°C


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
RTECS RF0760000
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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