核糖核酸酶A来源于牛胰腺 [RNA酶A],Ribonuclease A from bovine pancreas [RNase A];Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

别名:RNaseA;核糖核酸 3′-嘧啶寡核苷酸水解酶;核糖核酸酶 I;胰核糖核酸酶;RNase-A;Pancreatic Ribonuclease;Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

CAS号: 9001-99-4 EC 号: 3.1.27.5 (BRENDA, IUBMB) MDL编号: MFCD00132181 NACRES: NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): R5250
CAS号: 9001-99-4
MDL号: MFCD00132181
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
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说明

一般描述

RNase A(核糖核酸酶A)是一种内切核糖核酸酶,在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端(例如,pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG)。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同,并非糖蛋白。核糖核酸酶不会水解DNA,因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外,RNase也被DNA竞争性抑制。
Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.[1] The ribonuclease (RNase) gene is mapped to human chromosome 14.[2]
 

应用

  • RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
  • RNase A还用于RNA序列分析和保护测定。
  • RNase A已用作计算辅助药物设计的工具。
  • RNase A为RNA序列分析提供支持。
  • RNase A水解蛋白质样品中的RNA。
  • RNase A为DNA纯化提供支持。
Ribonuclease A from bovine pancreas has been used to treat human glioblastoma U251 cell line prior to harvesting, for flow cytometry analysis,[3] T suppressor cells[4] and embryonic stem cell chromatin.[5]
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. [6] Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins. [7]
 

生化/生理作用

The structure of Ribonuclease A (RNase A) comprises of four disulfide and catalytic triad of two histidines crucial for its functionality.[2] RNase A displays three-dimensional domain swapping of the α-helical N-terminal and the C-terminal β-strand. It can exist as dimer or oligomers.[8] The level of RNase is elevated in cancers and infectious diseases.[9] RNase A family of enzymes serve as potential drug targets.[2] The homolog and variants of RNase A are potential chemotherapeutic agents.[10]
 

特点和优势

我们高度稳定的核糖核酸酶A——RNase A,适合于RNA去除、RNA测序和DNA纯化。
 

外形

0.2M 磷酸钠缓冲溶液,pH 6.4
 

制备说明

R5503 的进一步色谱纯化得到的特纯 RNAse A
 

分析说明

蛋白测定方法:E.
通过 SDS-PAGE 测定 RNase A 纯度
 
 

属性

生物来源

bovine pancreas

质量水平

200

类型

Type X-A

测定

≥90% (SDS-PAGE)

形式

buffered aqueous solution

specific activity

≥70 Kunitz units/mg protein

分子量

~13,700

异质活性

protease, essentially free

储存温度

−20°C

 

安全信息

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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