核糖核酸酶A来源于牛胰腺 [RNA酶A]，Ribonuclease A from bovine pancreas [RNase A]；Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.[1] The ribonuclease (RNase) gene is mapped to human chromosome 14.[2]

 

Ribonuclease A from bovine pancreas has been used to treat human glioblastoma U251 cell line prior to harvesting, for flow cytometry analysis,[3] T suppressor cells[4] and embryonic stem cell chromatin.[5]

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. [6] Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins. [7]

 

The structure of Ribonuclease A (RNase A) comprises of four disulfide and catalytic triad of two histidines crucial for its functionality.[2] RNase A displays three-dimensional domain swapping of the α-helical N-terminal and the C-terminal β-strand. It can exist as dimer or oligomers.[8] The level of RNase is elevated in cancers and infectious diseases.[9] RNase A family of enzymes serve as potential drug targets.[2] The homolog and variants of RNase A are potential chemotherapeutic agents.[10]

 

我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。

 

0.2M 磷酸钠缓冲溶液，pH 6.4

 

R5503 的进一步色谱纯化得到的特纯 RNAse A

 

蛋白测定方法：E.

通过 SDS-PAGE 测定 RNase A 纯度