杜氏改良Eagle培养基 (高葡萄糖)，Dulbecco′s Modified Eagle′s Medium-high glucose [DMEM]；With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

 

Dulbecco′s Modified Eagle′s Medium (DMEM)是对Basal Medium Eagle (BME)的一种修改，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

 

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