曲拉通X100 (还原型)，Triton™ X-100 reduced

当 TX-100 的苯部分被完全氢化为环己烷衍生物时，形成还原的 Triton^™ X-100（RTX-100）。RTX-100 可能促进酶的消化，并且对细菌视紫红质的光异构化也有一定的影响。[1]

Triton^™ X-100 reduced is a versatile nonionic surfactant with a hydrophilic polyethylene oxide chain, widely utilized as a laboratory detergent. Its applications span various areas, including cell biology and biochemical research. This detergent is commonly employed for tasks such as cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a crucial component in lysis buffers. Triton^™ X-100 plays a key role in the isolation of lipid rafts, contributing to enhanced solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, Triton^™ X-100 exhibits excellent wetting properties and facilitates emulsification.

In biochemical and cell biology research, Triton^™ X-100 is instrumental in solubilizing membrane-bound proteins and isolating lipid rafts. Its unique properties allow for the preservation of the native conformation of proteins obtained from cellular membranes in solution. Triton^™ X-100 reduced is derived from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative. This modified version, RTX-100, has demonstrated potential in enhancing enzyme digestion and influencing the photoisomerization of bacteriorhodopsin, showcasing its versatility and utility in advanced research applications.

 

还原型 Triton^™ X-100 已用于：

• 作为 LB-TT 的一种成分，用于从大鼠脑中提取总蛋白[2]
• 在 ADP-Glo 测定和细胞磷酸腺苷三磷酸酶（ATP 酶）测定中[3]
• 在磷酸盐缓冲盐水（PBS）溶液中，用于成纤维细胞在 5′-乙炔-尿苷染色、免疫荧光和免疫标记中的透化[4]

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow