明胶 [来源于猪皮肤]，Gelatin from porcine skin；gel strength 80-120 g Bloom, Type A

建议将本产品用作1-5μg/cm2或0.5-50μg/mL的细胞培养基质。最佳浓度取决于细胞类型以及应用和研究目标。
明胶已在许多应用中使用。它用于涂层细胞培养，以改善细胞的附着，添加到PCR中以稳定Taq DNA，在Western blotting、ELISA和免疫化学中用作阻断剂，并在细菌学中用作物种分化介质的组成部分。作为一种生物相容性聚合物，它被用作释放活性生物分子的运载工具，并用于生成组织工程应用的支架。在制药工业中，明胶可作为悬浮剂和封装剂等应用。
干燥的明胶在室温下储存在密闭容器中时，将保持多年不变。当在100°C下在空气中加热时，它膨胀变软，并随着吡啶碱和氨的析出分解为碳质物质。
本产品来源于猪皮。明胶在热水中比在冷水中溶解。它几乎不溶于大多数有机溶剂，如酒精、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。布卢姆凝胶计测定的布卢姆数是已知浓度溶液形成的凝胶强度的指示。Bloom数与平均分子质量成正比。猪皮明胶的Bloom数从90克到300克不等。该产品的凝胶强度为80-120。

 

This product is recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

 

明胶存在于胶原蛋白中，是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

 

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

 

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 80-120.