抗体纯选耐碱蛋白A亲和逆流色谱树脂,MabSelect SuRe pcc protein A resin

短停留时间的高容量使 MabSelect SuRe pcc 蛋白 A 树脂成为连续加工的绝佳选择。17549101;17549102;17549103;17549105;17549104
货号(SKU): 17549103
增值税发票 √ 免费送货 √ 订货电话:010-68689484

The high capacity at short residence time makes MabSelect SuRe pcc protein A resin an excellent choice for continuous processing.

MabSelect SuRe pcc 蛋白 A 树脂是一种亲和层析树脂,用于纯化单克隆抗体和 Fc 融合蛋白。短停留时间下的高树脂容量使其成为单克隆抗体 (mAb) 纯化中定期逆流色谱的绝佳选择。碱性稳定的重组蛋白配体可实现高效的树脂清洁。

高动态结合容量(例如,约 60 g IgG/L 树脂,停留时间为 2.4 分钟)。
涵盖全面的供应安全计划,包括琼脂糖基质和蛋白 A 配体的双重来源。
在原位清洗 (CIP) 协议中使用的碱性条件下,碱稳定的蛋白 A 衍生配体使树脂比传统的基于蛋白 A 的树脂具有更高的稳定性。因此,使用具有成本效益的试剂(例如氢氧化钠 (NaOH))进行清洁的可能性将提高过程经济性。此外,使用 NaOH 进行清洁可以有效地灭活细菌、霉菌和酵母,从而提高产品质量。

MabSelect SuRe pcc 的基础基质结合了为单克隆抗体优化的孔隙率和提供良好压力/流动特性的刚性。这些功能使树脂能够以高流速运行,从而提高连续捕获步骤的生产率。小珠尺寸可实现双特异性抗体纯化等应用所需的高分辨率。

MabSelect SuRe pcc 是 Cytiva 定制设计树脂计划的一部分。

MabSelect SuRe pcc protein A resin is an affinity chromatography resin for purification of monoclonal antibody and Fc-fusion proteins. The high resin capacity at short residence times makes it an excellent choice for periodic counter-current chromatography in monoclonal antibody (mAb) purification. The alkaline-stable, recombinant protein ligand allows for efficient resin cleaning.

  • High dynamic binding capacity (e.g., ~ 60 g IgG/L resin at 2.4 min residence time).
  • High productivity through capture of large mass of monoclonal antibody in a short period of time.
  • Covered by a comprehensive security of supply program, including dual sources of the agarose base matrix and protein A ligand.

Resin cleaning advantage

The alkali-stabilized, protein A-derived ligand gives the resin greater stability than conventional protein A-based resins under the alkaline conditions used in cleaning-in-place (CIP) protocols. Consequently, the possibility of cleaning with cost-effective reagents such as sodium hydroxide (NaOH) will improve process economy. In addition, the use of NaOH for cleaning efficiently inactivates bacteria, mold, and yeast, with improved product quality as a result.

Increases productivity of continuous chromatography capture steps

The base matrix of MabSelect SuRe pcc combines a porosity optimized for monoclonal antibodies with a rigidity that delivers good pressure/flow properties. These features provide the ability to run the resin at high flow rates, thereby increasing productivity of continuous capture steps. The small bead size allows the high resolution required for applications such as purification of bispecific antibodies.

MabSelect SuRe pcc is part of Cytiva's program for custom designed resins.

Prepacked columns and other formats

MabSelect SuRe pcc is available in the following formats:

Process development scale – prepacked columns

Lab/research scale – prepacked for manual protein purification

HiTrap MabSelect SuRe pcc columns – for easy use with a syringe, peristaltic pump, or chromatography system.

Lab/research scale – prepacked columns for automated protein purification and analysis

HiTrap MabSelect SuRe pcc columns – for easy use with a syringe, peristaltic pump, or chromatography system.

For samples, contact your local sales representative or customer support.

MabSelect SuRe pcc protein A resin builds on the proven track record of MabSelect and MabSelect SuRe resins in downstream processing of monoclonal antibodies.

Learn about prepacked HiTrap MabSelect SuRe pcc and ReadyToProcess MabSelect SuRe pcc columns

ParameterMabSelect SuRe pcc, 1 L
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandAlkali-stabilized Protein A-derived (E. coli
Particle size, d50V1~50 µm 
MatrixHighly cross-linked agarose 
Ligand Coupling MethodEpoxy activation 
Binding Capacity/ml Chromatography Medium~ 60 mg human IgG/mL resin at 2.4 min residence time 
pH stability, operational23–12 
pH stability, CIP32–13.7 
Pressure/Flow SpecificationMinimum 250 cm/h. Determined in an AxiChrom 1000 column, bed height 20 cm, and operating pressure < 3 bar. Measured using process buffers with the same viscosity as water at 20°C 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in all aqueous buffers commonly used in Protein A Chromatography 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16