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抗体纯选耐碱蛋白A亲和树脂LX,MabSelect SuRe LX protein A resin

蛋白 A 层析树脂在延长的停留时间内具有非常高的动态结合能力,专为高滴度抗体纯化而开发。17547405;17547402;17547404;17547401;17547403
货号(SKU): 17547403
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 3,31,417.78

Protein A chromatography resin with very high dynamic binding capacity at extended residence times, developed for high-titer antibody purification.

MabSelect SuRe LX 是一种蛋白 A 亲和树脂,在延长的停留时间内具有非常高的动态结合能力。该层析树脂专为处理高滴度的单克隆抗体而开发。

新型耐碱 rProtein A 配体可承受 0.1 至 0.5 M NaOH 的严格就地清洁和消毒程序。
用于高滴度培养的高动态结合容量 (DBC) 减少了处理时间和树脂用量。
满足工业对供应安全、稳健性能和监管支持的需求。
非常适合生物过程应用中的抗体纯化
耐碱、高容量和低配体泄漏与刚性基础基质相结合,使 MabSelect SuRe LX 成为临床应用单克隆抗体纯化的绝佳选择。

MabSelect 蛋白 A 树脂家族的一部分
MabSelect SuRe LX 是蛋白 A 树脂 MabSelect 家族的成员,用于以工艺规模捕获单克隆抗体。 MabSelect SuRe LX 具有 MabSelect 和 MabSelect SuRe 的特性和优势,例如确保出色的压力/流动特性的刚性、高流速琼脂糖基质、导致洗脱液池中杂质水平低的非特异性结合以及定向配体偶联以实现出色的结合能力。它还具有与 MabSelect SuRe 相同的耐碱 rProtein A 配体。在原位清洁 (CIP) 和消毒方案中使用的碱性条件下,这种蛋白质配体比传统的基于蛋白质 A 的树脂具有更高的稳定性。

增强蛋白质配体的稳定性
MabSelect SuRe LX 增强的碱稳定性提高了工艺经济性和产品质量;可以使用具有成本效益的试剂(例如氢氧化钠)进行清洁,从而无需使用昂贵且危险的清洁剂(例如 Gua-HCl)。 MabSelect SuRe LX 增加的动态结合能力也提高了工艺经济性。

与 MabSelect SuRe 一样,MabSelect SuRe LX 的新型配体结构已被证明对蛋白酶具有更高的稳定性。因此,在洗脱过程中 MabSelect SuRe LX 配体的泄漏水平很低。

净化平台设计优势
MabSelect SuRe 已被证明可以为不同的单克隆抗体提供通用的洗脱条件,这些条件也适用于 MabSelect SuRe LX,因为它使用相同的配体。这是设计通用纯化平台流程时的优势。该特征还与 MabSelect SuRe 蛋白配体的构建相关。

MabSelect SuRe LX is a protein A affinity resin with very high dynamic binding capacity at extended residence times. The chromatography resin is developed for processing of monoclonal antibodies at high titers.

  • Novel, alkali-tolerant rProtein A ligand withstands rigorous clean-in-place and sanitization procedures with 0.1 to 0.5 M NaOH.
  • High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of resin used.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Excellent for antibody purification in bioprocess applications

Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matrix makes MabSelect SuRe LX an excellent choice for purification of monoclonal antibodies for clinical applications.

Part of the MabSelect protein A resin family

MabSelect SuRe LX is a member of the MabSelect family of protein A resins for the capture of monoclonal antibodies at process scale. MabSelect SuRe LX shares features and benefits of MabSelect and MabSelect SuRe such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for excellent binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect SuRe. This protein ligand provides greater stability than conventional protein A-based resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols.

Enhanced stability of the protein ligand

The enhanced alkali stability of MabSelect SuRe LX improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect SuRe LX.

Just as for MabSelect SuRe, the novel ligand construct of MabSelect SuRe LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect SuRe LX ligand during elution is low.

Purification platform design advantage

MabSelect SuRe has been demonstrated to give generic elution conditions for different monoclonal antibodies, which can be applied also to MabSelect SuRe LX as it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect SuRe protein ligand.

Prepacked columns and predictor filter plates.

MabSelect SuRe LX is available in the following formats:

Process development scale – prepacked columns and plates

  • PreDictor MabSelect SuRe LX – 96-well plates for high-throughput screening or process development.
  • PreDictor RoboColumn MabSelect Sure LX – miniaturized column format for high-throughput process development.
  • HiScreen MabSelect SuRe LX columns – optimized for method and process development.

Lab/research scale – prepacked columns for manual protein purification

HiTrap MabSelect SuRe LX chromatography columns – for easy use with a syringe, peristaltic pump, or chromatography system.

Lab/research scale – prepacked columns for automated protein purification and analysis

HiTrap MabSelect SuRe LX chromatography columns – for easy use with a syringe, peristaltic pump, or chromatography system.

Process scale protein purification – prepacked columns

ReadyToProcess MabSelect SuRe LX columns – 1 L and larger chromatography columns for clinical manufacturing that avoid the need for cleaning and cleaning validation.

For samples, contact your local sales representative or customer support.

MabSelect SuRe LX protein A resin builds on the proven track record of MabSelect and MabSelect SuRe resins in downstream processing of monoclonal antibodies.

Learn about prepacked HiScreen MabSelect SuRe LXReadyToProcess MabSelect SuRe LX columnsPreDictor MabSelect SuRe LX or PreDictor Robocolumns MabSelect SuRe LX

ParameterMabSelect SuRe LX, 1 L
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandAlkali-stabilized Protein A-derived (E. coli
Particle size, d50V1~85 µm 
MatrixHighly cross-linked agarose 
Ligand Coupling MethodEpoxy activation 
Binding Capacity/ml Chromatography Medium2Approximately 60 mg human IgG/mL resin. 
pH stability, operational33–12 
pH stability, CIP42–13.7 
Pressure/Flow Specification500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in all aqueous buffers commonly used in Protein A Chromatography 
Certificate of AnalysisYes 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2Determined at 10 % breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a column with a bed height of 10 cm, i.e. residence time is 6.0 min.
  • 3pH range where resin can be operated without significant change in function.
  • 4pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16