抗体纯选耐碱蛋白A亲和树脂,MabSelect SuRe antibody purification resin

MabSelect SuRe 的重组蛋白 A 配体经设计可耐受 0.1–0.5 M NaOH,从而能够有效清洁色谱树脂。17543803;17543804;17543805;17543801;17543802
货号(SKU): 17543803
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Protein A resin for efficient monoclonal antibody purification. This chromatography resin withstands 0.1–0.5 M NaOH.

在工艺规模的应用中,这种 NaOH 耐受性延长了树脂的使用寿命,并允许使用严格且具有成本效益的 CIP 和消毒协议。
在研究应用中,这种 NaOH 耐受性能够以最小化交叉污染风险来纯化多种抗体类型。
与未修饰的蛋白质 A 相比,专为增强蛋白酶抗性而设计的蛋白质配体可降低配体泄漏。
MabSelect SuRe 树脂特性
这种用于单克隆抗体 (mAb) 纯化的树脂包含刚性、高流量琼脂糖基质,可提供出色的压力/流量特性。与 Cytiva 的许多蛋白 A 层析树脂一样,MabSelect SuRe 使用定向配体偶联以获得出色的结合能力。它表现出较低的非特异性蛋白质结合,从而降低了洗脱液池中的杂质水平。

耐碱蛋白 A 配体的好处
MabSelect SuRe 树脂具有用于 mAb 纯化的工程化耐碱 rProtein A 配体。在原位清洁 (CIP) 和消毒方案中使用的碱性条件下,该配体比传统的蛋白质 A 亲和树脂具有更高的稳定性。该树脂可以承受使用 0.1 至 0.5 M NaOH 的严格 CIP 和消毒程序。这种树脂增强的碱稳定性提高了工艺经济性和产品质量;可以使用具有成本效益的试剂(例如氢氧化钠)进行清洁,从而无需使用昂贵且危险的清洁剂(例如 Gua-HCl)。

不同 mAb 的通用洗脱条件
MabSelect SuRe 树脂为不同的单克隆抗体提供通用的洗脱条件,这在设计纯化平台流程时是一个优势。该特征还与 rProtein A 配体的构建相关,该配体与人抗体中存在的 VH3 (Fab) 域的相互作用减少。

兼容的空柱 – 生物工艺规模
对于工艺规模的应用,Cytiva 建议将 AxiChrom 色谱柱用于 MabSelect 系列产品。

The recombinant protein A ligand of MabSelect SuRe has been engineered to withstand 0.1–0.5 M NaOH, enabling efficient cleaning of the chromatography resin.

  • In process-scale applications, this NaOH tolerance extends the lifetime of the resin and allows for the use of rigorous and cost-effective CIP and sanitization protocols.
  • In research applications, this NaOH tolerance enables the purification of multiple antibody types with minimized cross-contamination risk.
  • Protein ligand designed for enhanced protease resistance compared with unmodified protein A leads to lower ligand leakage.
  • Meets industrial demands in terms of comprehensive technical and regulatory support and security of supply.
  • Animal-component free raw materials and manufacturing process.

MabSelect SuRe resin properties

This resin for monoclonal antibody (mAb) purification contains a rigid, high-flow agarose matrix that delivers excellent pressure/flow properties. Like many of Cytiva's protein A chromatography resins, MabSelect SuRe uses oriented ligand coupling for excellent binding capacity. It exhibits low nonspecific protein binding, thereby decreasing impurity levels in the eluate pool.

Benefits of alkali-tolerant protein A ligand

MabSelect SuRe resin features an engineered, alkali-tolerant rProtein A ligand for mAb purification. The ligand provides greater stability than conventional protein A affinity resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The resin can withstand rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH. The enhanced alkali stability of this resin improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl.

Generic elution conditions for different mAbs

MabSelect SuRe resin offers generic elution conditions for different monoclonal antibodies, which is an advantage when designing purification platform processes. This feature is also correlated to the construction of the rProtein A ligand, which has a reduced interaction with the VH3 (Fab) domain present in human antibodies.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom chromatography columns for the MabSelect family of products.

Convenient prepacked columns – research scale

This chromatography resin is also available in the following formats:

  • HiTrap MabSelect SuRe: convenient for easy use with a syringe, peristaltic pump, and chromatography system
  • HiScreen MabSelect SuRe for method and process development
  • PreDictor MabSelect SuRe 96-well filter plate for high-throughput screening or process development
  • PredDictor RoboColumns MabSelect– miniaturized column format for high-throughput process development

Convenient prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns are available, eliminating the need for column packing and cleaning validation.

Learn about prepacked HiScreen MabSelect SuReHiTrap MabSelect SuReReadyToProcess MabSelect SuRe columnsPreDictor MabSelect SuRe or PreDictor Robocolumns MabSelect SuRe

ParameterMabSelect SuRe, 1 L
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandAlkali-stabilized Protein A-derived (E. coli
Particle size, d50V1~85 µm 
MatrixHighly cross-linked agarose, spherical 
Ligand Coupling MethodEpoxy activation 
Binding Capacity/ml Chromatography Medium2Approx. 35 mg human lgG/mL at 2.4 min residence time 
Dynamic binding capacity, QB103~35 mg hIgG/mL resin 
pH stability, operational43–12 
pH stability, CIP52–13.7 
Storage2 to 8°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol) 
Chemical stabilityStable in aqueous buffers commonly used in Protein A Chromatography 
Certificate of AnalysisYes 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
  • 3Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a XK 16/20 at 20 cm bed height (2.4 min residence time) for human IgG in 0.020 M NaH₂PO₄, pH 7.4
  • 4pH range where resin can be operated without significant change in function
  • 5pH range where resin can be subjected to cleaning-in-place without significant change in function
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16