异硫氰酸荧光素 [5(6)-异构体混合物]，Fluorescein 5(6)-isothiocyanate [FITC]；BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

图片 异硫氰酸荧光素 [5(6)-异构体混合物]，Fluorescein 5(6)-isothiocyanate [FITC]；BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
别名：荧光素 5(6)-异硫氰酸酯；异硫氰酸荧光素5,6混合异构体；FITC, Fluorescein isothiocyanate

Empirical Formula (Hill Notation): C21H11NO5S CAS号: 27072-45-3 分子量: 389.38 Beilstein: 1407295 EC 号: 248-207-7 MDL编号: MFCD00005063 PubChem化学物质编号: 329757273 NACRES: NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: 46950
CAS号: 27072-45-3
MDL号: MFCD00005063
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说明

荧光素5（6）-异硫氰酸酯，也称为FITC，是一种弱酸性羟基黄嘌呤，其总尺寸较小，在侧苯环上有羧酸和异硫氰酸盐取代基。该染料具有中等大小的非平面芳香体系。异硫氰酸酯基团与生物分子的亲核取代基（如氨基、羟基、硫醇）反应，作为荧光标记附着。在相同的溶剂中，染料的最大发射波长为519nm。
荧光素5（6）-异硫氰酸酯（FITC）标记的分子用于非显微镜免疫测定和生理示踪实验。FITC广泛用于流式细胞术实验，用于对蛋白质、细胞核和亚核颗粒进行染色。白细胞（WBCs）用FITC标记，以研究它们对化学引诱剂的反应，最终进行测量。

一般描述

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
 

应用

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
用于蛋白质的荧光标记（例如免疫荧光）;凝胶色谱中的 H 蛋白质标记
 
 

属性

质量水平

100

产品线

BioReagent

测定

≥90% (HPLC)

荧光

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

适用性

suitable for fluorescence

储存温度

−20°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

 

安全信息

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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