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异硫氰酸荧光素-葡聚糖,Fluorescein isothiocyanate–dextran [FITC–Dextran];average mol wt 40,000

别名:FITC标记葡聚糖;FITC-右旋糖酐;FITC-葡聚糖;异硫氰酸荧光素标记的葡聚糖;

CAS号 60842-46-8 MDL number MFCD00131092 NACRES NA.25
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): FD40S
CAS号: 60842-46-8
MDL号: MFCD00131092
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¥864.56

产品描述

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.[6][7] Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.[8] FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9. [5]

Application

Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:
• Fluorescein-dextran microscopy.[9][1][10]
• To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.[2]
• To quantify endocytic activity by flow cytometry.[3]
• To label α-lactalbumin and ribonuclease.[4]
FITC-dextran is suitable for extensive microcirculation and cell permeability research utilizing microfluorimetry. It is also suitable for the study of plant cell wall porosity and capillary permeability.

用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

包装

1 g in poly bottle

100, 250 mg in poly bottle

Linkage

与 FD-40 类似,但由 Sigma 生产。

Preparation Note

Sigma 通常以 25mg/ml 或更高的浓度测试 FITC 葡聚糖在水中的溶解度。 溶液应避光。 在体内,FITC-葡聚糖稳定超过 24 小时。

Other Notes

天然葡聚糖的分子量(MW)经证实在 900 万至 5 亿的范围内。较低分子量的葡聚糖将表现出略微较少的分支,并且具有更窄的分子量分布范围。分子量大于 10,000 的葡聚糖则表现为高度支化。 随着分子量增加,葡聚糖分子的对称性增强。 分子量为 2,000 至 10,000 的葡聚糖表现出可膨胀线圈的特性。 在分子量低于 2,000 时,葡聚糖更像棒状。通过以下一种或多种方法测量葡聚糖的分子量:低角度激光散射、尺寸排阻色谱法、铜络合和蒽酮试剂显色还原末端糖测定和粘度。

性质

Quality Level200
biological sourcesynthetic (organic)
formpowder
mol wtaverage mol wt 40,000
extent of labeling0.003-0.020 mol FITC per mol glucose
solubilityH2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
conjugateFITC conjugate
storage temp.2-8°C


安全信息

符号
GHS07  GHS07
信号词 Warning
危害声明 H315 - H319 - H335
靶器官 Respiratory system
个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 155 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 78 | 香港与北京 ≥ 32
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