尿酸酶来源于念珠菌属，Uricase from Candida sp.；recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

缺乏尿酸酶的小鼠出现尿酸肾病和尿崩症，重组尿酸酶治疗可缓解。 [1]

Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

 

Uricase from Candida sp. has been used:

• to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
• to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
• for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

 

在 25°C、pH 8.5 的条件下，1 个单位每分钟将把 1.0 μmol 尿酸氧化成尿囊素。

 

粉末；含柠檬酸盐和蔗糖