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天然蛋白A琼脂糖凝胶FF抗体纯化树脂,nProtein A Sepharose Fast Flow antibody purification resin

nProtein A Sepharose 4 Fast Flow是一种天然的蛋白质A树脂,用于纯化单克隆和多克隆抗体。
货号(SKU): 17528002
增值税发票 √ 免费送货 √ 订货电话:010-68689484
€785.46

nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies.

用于实验室和工艺规模的单克隆和多克隆抗体的纯化。
用于常规商业多克隆和单克隆抗体的纯化和生产。
不含动物源性成分。
非常适合免疫沉淀程序。
亲水性基础基质确保洗脱池中非特异性结合水平低,宿主细胞来源的杂质水平低。
满足工业对供应安全、稳健性能和监管支持的需求。
用于抗体纯化
nProtein A Sepharose 4 Fast Flow 是与成熟的 Sepharose 4 Fast Flow 基础基质偶联的天然蛋白质 A。它既可用于商业生产,也可用于研究目的。天然蛋白质对 IgG Fc 区的高度特异性提供了高纯化因子。

作为 BioProcess 培养基(树脂)系列的一员,nProtein A Sepharose 4 Fast Flow 通过供应安全性和全面的技术和法规支持满足工业需求。

在已发表的研究中成功使用
nProtein A Sepharose Fast Flow 用于许多应用的免疫沉淀程序,包括:

Sirtuin-1对STAT3抑制糖异生作用的研究
同源域转录因子sine oculis全基因组DNA结合模式的研究
兼容的空柱——研究规模
Cytiva 的解决方案包括用于填充您自己的色谱柱的各种空柱。 nProtein A Sepharose 4 Fast Flow 树脂与 Tricorn、XK 和 HiScale 色谱柱兼容。

兼容的空柱 – 生物工艺规模
对于工艺规模的应用,Cytiva 推荐使用 AxiChrom 色谱柱。 nProtein A Sepharose Fast Flow 也可以装入其他色谱柱平台,例如 BPG 和 Chromaflow。

  • For the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
  • Used in routine commercial polyclonal and monoclonal antibody purification and production.
  • Free from animal-derived components.
  • Well suited to immunoprecipitation procedures.
  • Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Use in antibody purification

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well-established Sepharose 4 Fast Flow base matrix. It is used for commercial production but also for research purposes.The high specificity of the native protein for the Fc region of IgG gives a high purification factor.

As a member of the BioProcess media (resins) range, nProtein A Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Successful use in published research

nProtein A Sepharose Fast Flow is used in immunoprecipitation procedures for many applications, including:

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for packing your own chromatography columns. nProtein A Sepharose 4 Fast Flow resin is compatible for use with Tricorn, XK and HiScale columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. nProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

 

ParameternProtein A Sepharose 4 Fast Flow
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
Particle size, d50V1~90 µm 
Matrixcross-linked agarose, 4%, spherical 
Ligand Coupling MethodCyanogen bromide activation 
Binding Capacity/ml Chromatography Medium>35 mg human IgG/mL 
Ligand density6 mg Protein A/ml medium 
Total binding capacity2≥30 mg hIgG/mL chromatography resin 
pH stability, operational33–9 
pH stability, CIP42–10 
Pressure/flow characteristics5150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water). 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in aqueous buffers commonly used in Protein A chromatography, 6 M guanidine-HCl, 70% ethanol, 3 M NaSCN, 0.1 M glycine (pH 3.0), 2% benzyl alcohol or 20 % ethanol 
Certificate of AnalysisYes 
  • 1Median particle size of the cumulative volume distribution.
  • 2Protein in excess is loaded in 0.020 M NaH₂PO₄ at pH 7 on a 7.5/50 PEEK-column. The binding capacity is obtained by measuring the amount of eluted protein in 0.1 M Glycine at pH 3.
  • 3pH range where resin can be operated without significant change in function. pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2.
  • 4pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function. pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2.
  • 5The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.

 

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16