Cytiva™ superSEC resin is a size exclusion chromatography (SEC) resin designed for purification of large biomolecules such as exosomes.
Cytiva™ super SEC 填料是我们定制设计培养基计划的一部分。它是琼脂糖™ CL-2B的替代品，后者可小规模纯化外泌体。Cytiva™ superSEC 填料适用于更高的流量，在 20 cm 床高 （<200 kPa） 下高达 300 cm/h。
Cytiva™ superSEC 填料基于琼脂糖基质，具有良好的压力流动特性和相对较大的孔隙率。孔径分布使填料适用于从较小尺寸的杂质（如宿主细胞蛋白和酶降解的 DNA）中分离外泌体和类似大小的实体。基质的特性使 Cytiva™ superSEC 填料成为大分子高生产率纯化的绝佳选择。树脂的亲水性可最大限度地减少非特异性吸附，并最大限度地回收所需的生物分子或实体。
概述
Cytiva™ superSEC resin is part of our Custom Designed Media program. It is an alternative to Sepharose™ CL-2B, which purifies exosomes at small scale. Cytiva™ superSEC resin is suitable for much higher flows, up to 300 cm/h at 20 cm bed height (<200 kPa).
- Efficient industrial-scale purification of large biomolecules based on our well-established platform of size exclusion chromatography (SEC) resins
- Flexible process design due to large operational window of flow rates and bed heights
- Shorter chromatography cycle time and, consequently, improved productivity compared with Sepharose CL-2B
- Excellent chemical stability
Cytiva™ superSEC resin is based on an agarose base matrix with good pressure-flow properties and relatively large porosity. The pore size distribution makes the resin suitable for fractionation of exosomes and similarly sized entities from smaller sized impurities, like host cell proteins and enzymatically degraded DNA. The features of the base matrix make Cytiva™ superSEC resin an excellent choice for high-productivity purification of large biomolecules. The hydrophilic nature of the resin minimizes nonspecific adsorption and maximizes recovery of the desired biomolecule or entity.
