sevenspikes.themes.common.filters
sevenspikes.themes.common.closemenu

复合模式多峰层析树脂,Capto Core 400

Capto Core 400 设计用于病毒和其他大生物分子的中间纯化和精制。17372401;17372404;17372402;17372403
货号(SKU): 17372403
增值税发票 √ 免费送货 √ 订货电话:010-68689484
€281.38

Capto Core 400 is designed for intermediate purification and polishing of viruses and other large biomolecules.

新型核心珠技术和多模式配体在一种色谱树脂中提供 Capto Core 400 双重功能、尺寸排阻和结合色谱。

与 SEC 相比,样品载量和流速显着提高了 100 倍,从而显着提高了生产率。
带有配体激活核心和非活性壳的珠子可以有效捕获杂质,同时在流通中收集目标分子。
由于流通色谱和强大的性能,实现了直接的优化,从而实现了广泛的操作窗口。
Capto Core 400 是尺寸排阻色谱 (SEC) 树脂的绝佳替代品,通常用于疫苗生产中病毒纯化的最后阶段。

了解预装 HiScreen Capto Core 400 和 HiTrap Capto Core 400 色谱柱或 Capto Core 700 树脂。

Novel core bead technology and multimodal ligand give Capto Core 400 dual functionality, size exclusion and binding chromatography, in one chromatography resin.

  • Significantly improved productivity enabled by up to 100‑fold higher sample load and significantly higher flow rates compared with SEC.
  • Bead with ligand‑activated core and inactive shell allows efficient capture of impurities, while target molecules are collected in the flowthrough.
  • Straightforward optimization due to flow-through chromatography and robust performance, allowing for a wide window of operation.

Capto Core 400 is an excellent alternative to size‑exclusion chromatography (SEC) resins that are typically employed in the final stages of virus purification in vaccine manufacture.

Learn about prepacked HiScreen Capto Core 400 and HiTrap Capto Core 400 columns or the Capto Core 700 resin.

 

ParameterCapto Core 400, 1 liter
BioProcess resinYes 
LigandOctylamine 
Particle size, d50V190 μm 
MatrixHighly cross‑linked agarose 
Ionic Capacity40-85 µmol/mL(Cl-) 
pH stability, operational23 to 13 
pH stability, CIP33 to 14 
Flow velocity700 cm/h 
Chemical stabilityAll commonly used aqueous buffers, 1 M sodium hydroxide (NaOH)§, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol. 
Avoid UsingOxidizing agents, citrate buffers 
Molecular Weight (MW) Cut-OffMr 400 000 
Binding capacity/mL chromatography resin422 mg ovalbumin/mL resin 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function. pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function. pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2.
  • 4Dynamic binding capacity measured at 10% breakthrough with a residence time of 3 min (200 cm/h) on HiScreen columns. The buffer was 20 mM Tris‑HCl, 150 mM NaCl, pH 7.5.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32