Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
• The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
• The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.
Agriculture fields: control pathogens.
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis
25 units in glass bottle
Chitinase serves as a biopesticide against several fungi and insects. This hydrolytic enzyme is capable of cleaving the glycosidic bonds in chitin.
几丁质酶是一种降解几丁质的 30 kDa（约）胞外酶复合物。甲壳素在 2 个酶促反应中降解为 N-乙酰-D-氨基葡萄糖。首先，甲壳素通过甲壳糊精酶-甲壳素酶，一种聚（1,4-β-[2-乙酰胺基-2-脱氧-D-葡萄糖苷]）-聚糖水解酶去除甲壳素上的壳二糖单元。第二次反应涉及 N-乙酰-葡糖胺酶-壳生物酶，将双糖裂解为 N-乙酰-D-葡糖胺的单体亚单位。这种酶可分为内切和外切柠檬酸酶。内切几丁质酶的活性包括在几丁质链中的内部点的随机切割。外切壳聚糖酶活性由渐进操作组成，从甲壳素的非还原端开始，释放壳二糖或 N-乙酰-氨基葡萄糖单位。 来自 T. viride 的几丁质分解酶是细胞外几丁质分解酶的混合物， 表现出外源和内源几丁质酶活性。发现主要活性为 N-乙酰基-β-氨基葡萄糖苷酶的活性。
Features and Benefits
Chitinase is an extracellular complex of enzymes that degrade chitin. It is a lytic enzyme suitable for fungal cell walls lysis.
在 2 小时测定中，在 25℃、pH 6.0 条件下，每小时 1 个单位将从甲壳素中释放出 1.0 mg N-乙酰 D -氨基葡萄糖。
1 个新的 1 小时单位 = 约 50 个旧的 48 小时单位。