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内切核酸酶来源于粘质沙雷氏菌,Benzonase® Nuclease, Purity > 99%

有效降低粘度并从蛋白质溶液中去除核酸;Endonuclease from Serratia marcescens;Effective viscosity reduction and removal of nucleic acids from protein solutions;70664-3;CAS号: 9025-65-4 EC 号: 3.1.30.2 (BRENDA, IUBMB) MDL编号: MFCD00131010
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): 70664
CAS号: 9025-65-4
MDL号: MFCD00131010
增值税发票 √ 免费送货 √ 订货电话:010-68689484
€316.50

说明

Benzonase® 核酸酶是一种来自粘质沙雷氏菌的基因工程核酸内切酶。 它降解所有形式的 DNA 和 RNA(单链、双链、线性和环状),同时没有蛋白水解活性。 它在广泛的条件下都有效,并具有极高的比活性。 该酶将核酸完全消化为 2 到 5 个碱基长(低于杂交极限)的 5'-单磷酸终止寡核苷酸,非常适合从重组蛋白中去除核酸,从而符合 FDA 的核酸污染指南。 Benzonase 快速水解核酸的能力使该酶成为降低粘度以减少加工时间和增加蛋白质产量的绝佳选择。 例如,酶与 BugBuster 和 PopCulture 蛋白质提取试剂兼容,因此可以与这些试剂一起添加,以消除粘度并从大肠杆菌提取物中去除核酸。

一般描述

Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster and PopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.

The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionicdetergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by > 150 mM monovalentcations, > 100 mM phosphate, > 100 mMammonium sulfate, or > 100 mM guanidine HCl.

Benzonase Nuclease is available inultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/l and at a high concentration (HC) of 250 U/l. Both preparations are free of detectable protease and have specific activity> 1 × 106 U/mg protein. The > 99% puritygrade is tested for endotoxins and contains< 0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20℃.



Total endotoxin:< 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
Effective viscosity reduction and removal of nucleic acids from protein solutions
 

包装

10 ku in Glass bottle
 

应用

用于去除蛋白样品中的核酸。
 

生化/生理作用

消化天然或热变性的DNA和RNA。
 

警告

Toxicity: Standard Handling (A)
 

单位定义

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
 

法律信息

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
 
 

属性

storage conditions

-20C

 

安全信息

WGK Germany

WGK 2

商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16